Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis | |
Gu, Wenhui1,2; Xie, Xiujun3; Gao, Shan1,2; Zhou, Wei4; Pan, Guanghua3; Wang, Guangce1; Wang, GC | |
2013-07-26 | |
发表期刊 | PLOS ONE |
ISSN | 1932-6203 |
卷号 | 8期号:7页码:e67028 |
文章类型 | Article |
摘要 | Both biomass dominated green vegetative cells (GV) and astaxanthin-dominated orange resting cells (OR) affect the final astaxanthin yield in industry. Examination of Haematococcus pluvialis revealed that the OR cells greatly varied from the GV cells at both cellular and subcellular levels. In particular, the thylakoid membranes in the OR were disassembled and fragmented. Furthermore, the OR conserved most of the photosynthetic pigments, with elevated concentrations of violaxanthin, antheraxanthin, and neoxanthin. Notably, moderate photosynthesis was detected in OR, even though most of the thylakoid membranes were disassembled, when compared with those in the GV. However, the energy distribution pattern between photosystem I and II (PSI and PSII) in the OR favored PSI, which was also confirmed by 77-K fluorescence. As zeaxanthin was not detected in the OR, we attribute the acclimation role to astaxanthin, instead of xanthophyll cycle. Additionally, proteomic-scale comparison analysis of thylakoids of the OR and GV indicated no photosynthetically remarkable variations. However, an extensive acclimation mechanism of H. pluvialis was proposed, in which proteins in thylakoid of GV were noted to be involved in biomass accumulation and those in OR were involved in stress response. Conclusions of the comparative analysis might provide some physiological background of OR for astaxanthin production by using H. pluvialis.; Both biomass dominated green vegetative cells (GV) and astaxanthin-dominated orange resting cells (OR) affect the final astaxanthin yield in industry. Examination of Haematococcus pluvialis revealed that the OR cells greatly varied from the GV cells at both cellular and subcellular levels. In particular, the thylakoid membranes in the OR were disassembled and fragmented. Furthermore, the OR conserved most of the photosynthetic pigments, with elevated concentrations of violaxanthin, antheraxanthin, and neoxanthin. Notably, moderate photosynthesis was detected in OR, even though most of the thylakoid membranes were disassembled, when compared with those in the GV. However, the energy distribution pattern between photosystem I and II (PSI and PSII) in the OR favored PSI, which was also confirmed by 77-K fluorescence. As zeaxanthin was not detected in the OR, we attribute the acclimation role to astaxanthin, instead of xanthophyll cycle. Additionally, proteomic-scale comparison analysis of thylakoids of the OR and GV indicated no photosynthetically remarkable variations. However, an extensive acclimation mechanism of H. pluvialis was proposed, in which proteins in thylakoid of GV were noted to be involved in biomass accumulation and those in OR were involved in stress response. Conclusions of the comparative analysis might provide some physiological background of OR for astaxanthin production by using H. pluvialis. |
学科领域 | Science & Technology - Other Topics |
DOI | 10.1371/journal.pone.0067028 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000322838900004 |
WOS关键词 | THYLAKOID MEMBRANE POLYPEPTIDES ; CYCLIC ELECTRON FLOW ; CHLAMYDOMONAS-REINHARDTII ; LIFE-CYCLE ; SDS-PAGE ; ASTAXANTHIN ; CHLOROPHYCEAE ; FLUORESCENCE ; ELECTROPHORESIS ; IDENTIFICATION |
WOS标题词 | Science & Technology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/16548 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Wang, GC |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China 4.Marine Fisheries Inst Jiangsu, Nantong, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Gu, Wenhui,Xie, Xiujun,Gao, Shan,et al. Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis[J]. PLOS ONE,2013,8(7):e67028. |
APA | Gu, Wenhui.,Xie, Xiujun.,Gao, Shan.,Zhou, Wei.,Pan, Guanghua.,...&Wang, GC.(2013).Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis.PLOS ONE,8(7),e67028. |
MLA | Gu, Wenhui,et al."Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis".PLOS ONE 8.7(2013):e67028. |
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