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Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis
Gu, Wenhui1,2; Xie, Xiujun3; Gao, Shan1,2; Zhou, Wei4; Pan, Guanghua3; Wang, Guangce1; Wang, GC
2013-07-26
发表期刊PLOS ONE
ISSN1932-6203
卷号8期号:7页码:e67028
文章类型Article
摘要Both biomass dominated green vegetative cells (GV) and astaxanthin-dominated orange resting cells (OR) affect the final astaxanthin yield in industry. Examination of Haematococcus pluvialis revealed that the OR cells greatly varied from the GV cells at both cellular and subcellular levels. In particular, the thylakoid membranes in the OR were disassembled and fragmented. Furthermore, the OR conserved most of the photosynthetic pigments, with elevated concentrations of violaxanthin, antheraxanthin, and neoxanthin. Notably, moderate photosynthesis was detected in OR, even though most of the thylakoid membranes were disassembled, when compared with those in the GV. However, the energy distribution pattern between photosystem I and II (PSI and PSII) in the OR favored PSI, which was also confirmed by 77-K fluorescence. As zeaxanthin was not detected in the OR, we attribute the acclimation role to astaxanthin, instead of xanthophyll cycle. Additionally, proteomic-scale comparison analysis of thylakoids of the OR and GV indicated no photosynthetically remarkable variations. However, an extensive acclimation mechanism of H. pluvialis was proposed, in which proteins in thylakoid of GV were noted to be involved in biomass accumulation and those in OR were involved in stress response. Conclusions of the comparative analysis might provide some physiological background of OR for astaxanthin production by using H. pluvialis.; Both biomass dominated green vegetative cells (GV) and astaxanthin-dominated orange resting cells (OR) affect the final astaxanthin yield in industry. Examination of Haematococcus pluvialis revealed that the OR cells greatly varied from the GV cells at both cellular and subcellular levels. In particular, the thylakoid membranes in the OR were disassembled and fragmented. Furthermore, the OR conserved most of the photosynthetic pigments, with elevated concentrations of violaxanthin, antheraxanthin, and neoxanthin. Notably, moderate photosynthesis was detected in OR, even though most of the thylakoid membranes were disassembled, when compared with those in the GV. However, the energy distribution pattern between photosystem I and II (PSI and PSII) in the OR favored PSI, which was also confirmed by 77-K fluorescence. As zeaxanthin was not detected in the OR, we attribute the acclimation role to astaxanthin, instead of xanthophyll cycle. Additionally, proteomic-scale comparison analysis of thylakoids of the OR and GV indicated no photosynthetically remarkable variations. However, an extensive acclimation mechanism of H. pluvialis was proposed, in which proteins in thylakoid of GV were noted to be involved in biomass accumulation and those in OR were involved in stress response. Conclusions of the comparative analysis might provide some physiological background of OR for astaxanthin production by using H. pluvialis.
学科领域Science & Technology - Other Topics
DOI10.1371/journal.pone.0067028
URL查看原文
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000322838900004
WOS关键词THYLAKOID MEMBRANE POLYPEPTIDES ; CYCLIC ELECTRON FLOW ; CHLAMYDOMONAS-REINHARDTII ; LIFE-CYCLE ; SDS-PAGE ; ASTAXANTHIN ; CHLOROPHYCEAE ; FLUORESCENCE ; ELECTROPHORESIS ; IDENTIFICATION
WOS标题词Science & Technology
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16548
专题实验海洋生物学重点实验室
通讯作者Wang, GC
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China
4.Marine Fisheries Inst Jiangsu, Nantong, Peoples R China
第一作者单位中国科学院海洋研究所
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Gu, Wenhui,Xie, Xiujun,Gao, Shan,et al. Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis[J]. PLOS ONE,2013,8(7):e67028.
APA Gu, Wenhui.,Xie, Xiujun.,Gao, Shan.,Zhou, Wei.,Pan, Guanghua.,...&Wang, GC.(2013).Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis.PLOS ONE,8(7),e67028.
MLA Gu, Wenhui,et al."Comparison of Different Cells of Haematococcus pluvialis Reveals an Extensive Acclimation Mechanism during its Aging Process: From a Perspective of Photosynthesis".PLOS ONE 8.7(2013):e67028.
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