Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity

doi:10.1016/j.fsi.2012.11.039

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	Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity
	Jia, Pan-pan 1,2; Hu, Yong-hua 2; Chi, Heng 2; Sun, Bo-guang2 ; Yu, Wen-gong 1; Sun, Li 2; Sun, L
	2013-02-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	34 期号:2 页码:514-520
文章类型	Article
摘要	Vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. The genome of V anguillarum contains five flagellin genes, i.e. flaA,flaB,flaC,flaD, and flaE. In this study, we analyzed the vaccine potential and adjuvanticity of FlaA, FlaB, FlaD, and FlaE in a model of Japanese flounder (Paralichthys olivaceus). For this purpose, recombinant FlaA, FlaB, FlaD, and FlaE were expressed in and purified from Escherichia coli. In vivo immunogenicity analysis showed that antibodies against rFlaA, rFlaB, rFlaD, and rFlaE were detected in rat antiserum raised against live V. anguillarum, with the highest antibody level being that against rFlaB. When administered into flounder via intraperitoneal injection, rFlaA, rFlaD, and rFlaE induced comparable relative percent survival (RPS) rates, which were significantly lower than that induced by rFlaB. Specific serum antibodies were induced by all flagellins, however, the antibody level induced by rFlaB was significantly higher than those induced by other three flagellins. Compared to sera from fish vaccinated with rFlaA, rFlaD, and rFlaE, serum from fish vaccinated with rFlaB significantly reduced the infectivity of V. anguillarum against host cells. To examine the potential adjuvant effect of the flagellins, flounder were immunized with rEsa1, a D15-like surface antigen that induces protective immunity as a subunit vaccine, in the presence or absence of rFlaA, rFlaB, rFlaD, and rFlaE respectively. The results showed that rFlaE, but not other three flagellins, significantly increased the RPS of rEsa1. Compared to fish vaccinated with rEsa1, fish vaccinated with rEsa1 plus rFlaE exhibited a significantly higher level of serum antibodies and enhanced expression of the genes involved in innate and adaptive immunity. Taken together, these results indicate that FlaA, FlaB, FlaD, and FlaE have different immunological properties and, as a result, differ in vaccine and adjuvant potentials. (C) 2012 Elsevier Ltd. All rights reserved.; Vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. The genome of V anguillarum contains five flagellin genes, i.e. flaA,flaB,flaC,flaD, and flaE. In this study, we analyzed the vaccine potential and adjuvanticity of FlaA, FlaB, FlaD, and FlaE in a model of Japanese flounder (Paralichthys olivaceus). For this purpose, recombinant FlaA, FlaB, FlaD, and FlaE were expressed in and purified from Escherichia coli. In vivo immunogenicity analysis showed that antibodies against rFlaA, rFlaB, rFlaD, and rFlaE were detected in rat antiserum raised against live V. anguillarum, with the highest antibody level being that against rFlaB. When administered into flounder via intraperitoneal injection, rFlaA, rFlaD, and rFlaE induced comparable relative percent survival (RPS) rates, which were significantly lower than that induced by rFlaB. Specific serum antibodies were induced by all flagellins, however, the antibody level induced by rFlaB was significantly higher than those induced by other three flagellins. Compared to sera from fish vaccinated with rFlaA, rFlaD, and rFlaE, serum from fish vaccinated with rFlaB significantly reduced the infectivity of V. anguillarum against host cells. To examine the potential adjuvant effect of the flagellins, flounder were immunized with rEsa1, a D15-like surface antigen that induces protective immunity as a subunit vaccine, in the presence or absence of rFlaA, rFlaB, rFlaD, and rFlaE respectively. The results showed that rFlaE, but not other three flagellins, significantly increased the RPS of rEsa1. Compared to fish vaccinated with rEsa1, fish vaccinated with rEsa1 plus rFlaE exhibited a significantly higher level of serum antibodies and enhanced expression of the genes involved in innate and adaptive immunity. Taken together, these results indicate that FlaA, FlaB, FlaD, and FlaE have different immunological properties and, as a result, differ in vaccine and adjuvant potentials. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Vibrio Anguillarum Flagellin Vaccine Adjuvant
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2012.11.039
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000315076100012
WOS关键词	MUCOSAL IMMUNE-RESPONSES ; TOLL-LIKE RECEPTOR-5 ; FISH CELL-LINE ; BACTERIAL FLAGELLIN ; PARALICHTHYS-OLIVACEUS ; INNATE IMMUNITY ; EXPRESSION ; MOTILITY ; PROTEIN ; SALMONELLA
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16547
专题	实验海洋生物学重点实验室
通讯作者	Sun, L
作者单位	1.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Jia, Pan-pan,Hu, Yong-hua,Chi, Heng,et al. Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):514-520.
APA	Jia, Pan-pan.,Hu, Yong-hua.,Chi, Heng.,Sun, Bo-guang.,Yu, Wen-gong.,...&Sun, L.(2013).Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity.FISH & SHELLFISH IMMUNOLOGY,34(2),514-520.
MLA	Jia, Pan-pan,et al."Comparative study of four flagellins of Vibrio anguillarum: Vaccine potential and adjuvanticity".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):514-520.

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