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Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China
Xiao, Lin1,2,3; Liu, Haizhou1; Wu, Ning1; Liu, Ming1; Wei, Jianteng1; Zhang, Yuyan1; Lin, Xiukun1; Lin, XK
2013
发表期刊WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN0959-3993
卷号29期号:1页码:11-17
文章类型Article
摘要A moderately halophilic fungus F1 was isolated from a marine solar saltern in Weihai, China. The identification of the fungus F1 was performed by the morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on ITS (internal transcribed spacer)-5.8S rDNA region sequence comparison. The strain was identified as belonging to the genus Aspergillus and designated as Aspergillus sp. nov. F1. Furthermore, Aspergillus sp. nov. F1 grew well in 3-15 % (w/v) NaCl, and with increasing of salinity, the generation of secondary metabolites with cytotoxicity was also augmented. Three compounds with cytotoxicity were isolated from the ethyl acetate extract of the whole broth and mycelia of Aspergillus sp. nov. F1, and identified as ergosterol, rosellichalasin and cytochalasin E, respectively. Especially, ergosterol showed high potent cytotoxic activity to human colon cancer cell line RKO with IC50 of 3.3 +/- A 0.5 mu M. Considering the high cytochalasin production and the simple and economical fermentation of Aspergillus sp. nov. F1, the strain could be used as potential strain for large scale production of the cytochalasin E and rosellichalasin.; A moderately halophilic fungus F1 was isolated from a marine solar saltern in Weihai, China. The identification of the fungus F1 was performed by the morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on ITS (internal transcribed spacer)-5.8S rDNA region sequence comparison. The strain was identified as belonging to the genus Aspergillus and designated as Aspergillus sp. nov. F1. Furthermore, Aspergillus sp. nov. F1 grew well in 3-15 % (w/v) NaCl, and with increasing of salinity, the generation of secondary metabolites with cytotoxicity was also augmented. Three compounds with cytotoxicity were isolated from the ethyl acetate extract of the whole broth and mycelia of Aspergillus sp. nov. F1, and identified as ergosterol, rosellichalasin and cytochalasin E, respectively. Especially, ergosterol showed high potent cytotoxic activity to human colon cancer cell line RKO with IC50 of 3.3 +/- A 0.5 mu M. Considering the high cytochalasin production and the simple and economical fermentation of Aspergillus sp. nov. F1, the strain could be used as potential strain for large scale production of the cytochalasin E and rosellichalasin.
关键词Moderately Halophilic Fungus Aspergillus Cytotoxic Activity Cytochalasin
学科领域Biotechnology & Applied Microbiology
DOI10.1007/s11274-012-1152-9
URL查看原文
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000312075300002
WOS关键词SEQUENCE ALIGNMENT ; METABOLITE ; EXTRACTS ; BACTERIA ; HALOCINS ; GROWTH ; ACTIN ; CELLS
WOS标题词Science & Technology ; Life Sciences & Biomedicine
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16539
专题实验海洋生物学重点实验室
通讯作者Lin, XK
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China
第一作者单位中国科学院海洋研究所
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Xiao, Lin,Liu, Haizhou,Wu, Ning,et al. Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(1):11-17.
APA Xiao, Lin.,Liu, Haizhou.,Wu, Ning.,Liu, Ming.,Wei, Jianteng.,...&Lin, XK.(2013).Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(1),11-17.
MLA Xiao, Lin,et al."Characterization of the high cytochalasin E and rosellichalasin producing-Aspergillus sp. nov. F1 isolated from marine solar saltern in China".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.1(2013):11-17.
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