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Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration
Yue, Shidong1,2,3; Zhang, Yu1,2,3; Zhou, Yi1,2,4; Xu, Shaochun1,2,3; Xu, Shuai1,2,3; Zhang, Xiaomei1,2,4; Gu, Ruiting1,2,3
2019-12-26
Source PublicationPLANT METHODS
Volume15Issue:1Pages:10
AbstractBackground: Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results: Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as -5 degrees C. However, our results indicated that reduced storage temperature to 0 degrees C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 degrees C under a salinity of 40-60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 degrees C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 +/- 16.8%. Conclusion: Our experiments demonstrated that seeds stored at 0 degrees C under a salinity of 40-60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.
KeywordSeagrass Zostera japonica Seed Storage condition Temperature Salinity Desiccation sensitivity Conservation Restoration
DOI10.1186/s13007-019-0541-6
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2019YFD0901302] ; National Science & Technology Basic Work Program[2015FY110600] ; key Research and Development Project of Shandong Province[2017GHY15111] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; National Natural Science Foundation of China[41606192/41176140] ; Key Research Project of Frontier Sciences of CAS[QYZDB-SSW-DQC041-1] ; Marine Engineering Standardization Construction Project[CCSOA-OEAS-2018-13] ; Taishan Scholars Program (Distinguished Taishan Scholars) ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemical Research Methods ; Plant Sciences
WOS IDWOS:000510638800002
PublisherBMC
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/165395
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhou, Yi
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
Yue, Shidong,Zhang, Yu,Zhou, Yi,et al. Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration[J]. PLANT METHODS,2019,15(1):10.
APA Yue, Shidong.,Zhang, Yu.,Zhou, Yi.,Xu, Shaochun.,Xu, Shuai.,...&Gu, Ruiting.(2019).Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration.PLANT METHODS,15(1),10.
MLA Yue, Shidong,et al."Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration".PLANT METHODS 15.1(2019):10.
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