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Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain
Bi, Yunchen1,2,3; Zimmer, Jochen1
2020-02-04
发表期刊STRUCTURE
ISSN0969-2126
卷号28期号:2页码:252-+
通讯作者Zimmer, Jochen(jochen_zimmer@virginia.edu)
摘要A hallmark of Gram-negative bacteria is an asymmetric outer membrane containing lipopolysaccharides (LPSs) in the extracellular leaflet. LPS molecules consist of lipid A, which is connected to the inner and outer core oligosaccharides. This LPS core structure is extended in the periplasm by the O antigen, a variable and serotype-defining polysaccharide. In the ABC transporter-dependent LPS biosynthesis pathway, the WzmWzt transporter secretes the complete O antigen across the inner membrane for ligation to the LPS core. In some O antigen transporters, the nucleotide-binding domain of Wzt is fused C-terminally to a carbohydrate-binding domain (CBD) that interacts with the O antigen chain. Here, we present the crystal structure of the Aquifex aeolicus CBD that reveals a conserved flat and a variable twisted jelly-roll surface. The CBD dimer is stabilized by mutual beta strand exchange. Microbial glycan array binding studies with the isolated CBD provide insights into its interaction with complex carbohydrates.
DOI10.1016/j.str.2019.11.020
收录类别SCI
语种英语
资助项目NIH[1R01GM129666] ; Center for Functional Glycomics[R24 GM098791] ; DOE Office of Science[DE-SC0012704] ; National Center for Functional Glycomics[P41 GM103694]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS类目Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS记录号WOS:000512394400013
出版者CELL PRESS
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/165281
专题实验海洋生物学重点实验室
通讯作者Zimmer, Jochen
作者单位1.Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, Charlottesville, VA 22903 USA
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位中国科学院海洋研究所
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Bi, Yunchen,Zimmer, Jochen. Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain[J]. STRUCTURE,2020,28(2):252-+.
APA Bi, Yunchen,&Zimmer, Jochen.(2020).Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain.STRUCTURE,28(2),252-+.
MLA Bi, Yunchen,et al."Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain".STRUCTURE 28.2(2020):252-+.
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