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Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain
Bi, Yunchen1,2,3; Zimmer, Jochen1
2020-02-04
Source PublicationSTRUCTURE
ISSN0969-2126
Volume28Issue:2Pages:252-+
Corresponding AuthorZimmer, Jochen(jochen_zimmer@virginia.edu)
AbstractA hallmark of Gram-negative bacteria is an asymmetric outer membrane containing lipopolysaccharides (LPSs) in the extracellular leaflet. LPS molecules consist of lipid A, which is connected to the inner and outer core oligosaccharides. This LPS core structure is extended in the periplasm by the O antigen, a variable and serotype-defining polysaccharide. In the ABC transporter-dependent LPS biosynthesis pathway, the WzmWzt transporter secretes the complete O antigen across the inner membrane for ligation to the LPS core. In some O antigen transporters, the nucleotide-binding domain of Wzt is fused C-terminally to a carbohydrate-binding domain (CBD) that interacts with the O antigen chain. Here, we present the crystal structure of the Aquifex aeolicus CBD that reveals a conserved flat and a variable twisted jelly-roll surface. The CBD dimer is stabilized by mutual beta strand exchange. Microbial glycan array binding studies with the isolated CBD provide insights into its interaction with complex carbohydrates.
DOI10.1016/j.str.2019.11.020
Indexed BySCI
Language英语
Funding ProjectNIH[1R01GM129666] ; Center for Functional Glycomics[R24 GM098791] ; DOE Office of Science[DE-SC0012704] ; National Center for Functional Glycomics[P41 GM103694]
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Biophysics ; Cell Biology
WOS IDWOS:000512394400013
PublisherCELL PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165281
Collection实验海洋生物学重点实验室
Corresponding AuthorZimmer, Jochen
Affiliation1.Univ Virginia, Dept Mol Physiol & Biol Phys, Sch Med, Charlottesville, VA 22903 USA
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Bi, Yunchen,Zimmer, Jochen. Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain[J]. STRUCTURE,2020,28(2):252-+.
APA Bi, Yunchen,&Zimmer, Jochen.(2020).Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain.STRUCTURE,28(2),252-+.
MLA Bi, Yunchen,et al."Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain".STRUCTURE 28.2(2020):252-+.
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