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Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress
Li, Ao1,2,3,4; Li, Li1,2,3,5; Wang, Wei1,2,3,5; Zhang, Guofan1,3,5
2020-01-29
Source PublicationMARINE BIOTECHNOLOGY
ISSN1436-2228
Pages13
Corresponding AuthorLi, Li(lili@qdio.ac.cn)
AbstractLysine acetylation of proteins is a highly conserved post-translational modification that plays an important regulatory role in almost every aspect of metabolic processes in both terrestrial and aquatic species. Pacific oyster, Crassostrea gigas, a model marine species, is distributed worldwide and is economically and ecologically important. However, little is known about the role of acetylation in the adaptive response of oyster to heterogeneous intertidal environments. Here, we conducted the first-ever lysine acetylome analysis in two genetically and physiologically differentiated oyster populations, using a highly sensitive immune-affinity purification and high-resolution mass spectrometry. Overall, we identified 1054 lysine acetylation sites in 664 proteins, which account for 2.37% of the oyster proteome analysed in the current study. The modified proteins are involved in a wide range of biological processes and are localised in multiple cellular compartments. Motif analysis revealed that hydrophilic and polar amino acids histidine, lysine and arginine were the most enriched residues in the positions + 1 and + 2 of the acetylated sites. Further, the two oyster populations exhibited divergent acetylomic regulations of several biological pathways, particularly energy metabolism and glycine and serine amino acid metabolism, in response to thermal stress and differentiated acetylation patters of candidate heat-responsive proteins, e.g. molecular chaperone and myosin. These observations suggest that lysine acetylation plays a critical role in different thermal responses of these two oyster populations. These findings provide an important resource for in-depth exploration of the physiological role of lysine acetylation in adaptive evolution of marine invertebrates.
KeywordAcetylome Posttranslational modification Adaptive divergence Oyster Climate change
DOI10.1007/s10126-020-09947-6
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900304] ; China Postdoctoral Science Foundation[2019TQ0324] ; National Natural Science Foundation of China[31572620] ; Modern Agro-industry Technology Research System[CARS-49]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000510084600001
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165278
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Key Lab Ecol Mariculture, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Ao,Li, Li,Wang, Wei,et al. Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress[J]. MARINE BIOTECHNOLOGY,2020:13.
APA Li, Ao,Li, Li,Wang, Wei,&Zhang, Guofan.(2020).Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress.MARINE BIOTECHNOLOGY,13.
MLA Li, Ao,et al."Acetylome Analysis Reveals Population Differentiation of the Pacific Oyster Crassostrea gigas in Response to Heat Stress".MARINE BIOTECHNOLOGY (2020):13.
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