Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| An I kappa B homologue (FcCactus) in Chinese shrimp Fenneropenaeus chinensis | |
Wang, Dongdong1,2; Li, Fuhua1; Li, Shihao1,2; Chi, Yanhong1,2; Wen, Rong1,2; Feng, Ningning1,2; Xiang, Jianhai1 ; Li, FH
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| 2013-04-01 | |
| 发表期刊 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| 卷号 | 39期号:4页码:352-362 |
| 文章类型 | Article |
| 摘要 | This study firstly reports the characterization of a functional licB homologue, FcCactus in Chinese shrimp Fenneropenaeus chinensis. The full length cDNA of FcCactus consists of a 1359 bp open reading frame (ORF) encoding a 453 amino acid protein with a predicted molecular weight (MW) of 48.46 kDa and theoretical pl of 5.23. Phylogenetic analysis and multiple alignments revealed that the deduced amino acid sequence of FcCactus cDNA had high similarities to Cactus or licB reported in seven other arthropods. Genomic DNA sequence of FcCactus was also obtained with a length of more than 17698 bp and constituted of seven exons and six introns. Analysis on 5'-upstream regulatory region of its DNA sequence revealed that it contained the core promoter sequence with the TATA-box and transcription start site existing in it; furthermore, various transcription factor binding sites (HSF, Hb, BR-C Z, Dfd, CF2-II, Croc, Ttk, Dorsal, and c-Rel) were predicted. Spatial expression profiles showed that FcCactus mRNA had the highest expression level in muscle, hemocytes, heart and lymphoid organ. Gram-positive bacteria (Micrococcus lysodeikticus) and Gram-negative bacteria (Vibrio anguillarium) injection to shrimp caused the modulation of FcCactus at the transcription level. DsRNAi (double-strand RNA interference) approach was used to study the function of FcCactus and the data showed that FcCactus could regulate the expression of different antimicrobial peptides (AMPs) and antiviral factor (AV). The present data showed that FcCactus might play important roles in regulating the immune response of shrimp. (C) 2012 Elsevier Ltd. All rights reserved.; This study firstly reports the characterization of a functional licB homologue, FcCactus in Chinese shrimp Fenneropenaeus chinensis. The full length cDNA of FcCactus consists of a 1359 bp open reading frame (ORF) encoding a 453 amino acid protein with a predicted molecular weight (MW) of 48.46 kDa and theoretical pl of 5.23. Phylogenetic analysis and multiple alignments revealed that the deduced amino acid sequence of FcCactus cDNA had high similarities to Cactus or licB reported in seven other arthropods. Genomic DNA sequence of FcCactus was also obtained with a length of more than 17698 bp and constituted of seven exons and six introns. Analysis on 5'-upstream regulatory region of its DNA sequence revealed that it contained the core promoter sequence with the TATA-box and transcription start site existing in it; furthermore, various transcription factor binding sites (HSF, Hb, BR-C Z, Dfd, CF2-II, Croc, Ttk, Dorsal, and c-Rel) were predicted. Spatial expression profiles showed that FcCactus mRNA had the highest expression level in muscle, hemocytes, heart and lymphoid organ. Gram-positive bacteria (Micrococcus lysodeikticus) and Gram-negative bacteria (Vibrio anguillarium) injection to shrimp caused the modulation of FcCactus at the transcription level. DsRNAi (double-strand RNA interference) approach was used to study the function of FcCactus and the data showed that FcCactus could regulate the expression of different antimicrobial peptides (AMPs) and antiviral factor (AV). The present data showed that FcCactus might play important roles in regulating the immune response of shrimp. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Cactus Amps Av Immunity Fenneropenaeus Chinensis |
| 学科领域 | Immunology ; Zoology |
| DOI | 10.1016/j.dci.2012.12.005 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Immunology ; Zoology |
| WOS类目 | Immunology ; Zoology |
| WOS记录号 | WOS:000317248900005 |
| WOS关键词 | ANTIMICROBIAL PEPTIDE GENES ; NUCLEAR EXPORT SIGNAL ; TOLL RECEPTOR ; DNA-BINDING ; LITOPENAEUS-VANNAMEI ; PENAEUS-MONODON ; EXPRESSION PROFILES ; CRASSOSTREA-GIGAS ; WHITE SHRIMP ; TIGER SHRIMP |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16524 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Li, FH |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Dongdong,Li, Fuhua,Li, Shihao,et al. An I kappa B homologue (FcCactus) in Chinese shrimp Fenneropenaeus chinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,39(4):352-362. |
| APA | Wang, Dongdong.,Li, Fuhua.,Li, Shihao.,Chi, Yanhong.,Wen, Rong.,...&Li, FH.(2013).An I kappa B homologue (FcCactus) in Chinese shrimp Fenneropenaeus chinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,39(4),352-362. |
| MLA | Wang, Dongdong,et al."An I kappa B homologue (FcCactus) in Chinese shrimp Fenneropenaeus chinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 39.4(2013):352-362. |
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