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A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells
Wang, Feng-xia1,2; Wu, Ning3; Wei, Jian-teng4,5; Liu, Jia3; Zhao, Jin3; Ji, Ai-guo1; Lin, Xiu-kun3,6; Lin, XK
2013-08-01
发表期刊BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
ISSN0829-8211
卷号91期号:4页码:244-251
文章类型Article
摘要Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.; Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.
关键词Anticancer Protein Antimetastatic Activity Eupolyphaga Sinensis Beta 1-integrin Lung Cancer A549 Cells
学科领域Biochemistry & Molecular Biology ; Cell Biology
DOI10.1139/bcb-2013-0002
URL查看原文
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
WOS类目Biochemistry & Molecular Biology ; Cell Biology
WOS记录号WOS:000321891600008
WOS关键词PHASE-II ; CILENGITIDE ; METASTASIS ; INTEGRINS ; ANTIBODY ; GROWTH ; VENOM
WOS标题词Science & Technology ; Life Sciences & Biomedicine
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16516
专题实验海洋生物学重点实验室
通讯作者Lin, XK
作者单位1.Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China
2.Shandong Acad Sci, Inst Biol Res, Jinan 250014, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China
5.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China
6.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
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Wang, Feng-xia,Wu, Ning,Wei, Jian-teng,et al. A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells[J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,2013,91(4):244-251.
APA Wang, Feng-xia.,Wu, Ning.,Wei, Jian-teng.,Liu, Jia.,Zhao, Jin.,...&Lin, XK.(2013).A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells.BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,91(4),244-251.
MLA Wang, Feng-xia,et al."A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells".BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 91.4(2013):244-251.
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