A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens

doi:10.1016/j.dci.2013.03.005

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	A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens
	Zhang, Min 1; Long, Hao1,2 ; Sun, Li 1; Sun, L
	2013-07-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	40 期号:3-4 页码:258-265
文章类型	Article
摘要	NK-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. Mammalian NK-lysin is known to possess antibacterial property and antitumor activity. Homologues of NK-lysin have been identified in several teleost species, but the natural function of fish NK-lysin remains essentially unknown. In this study, we identified a NK-lysin, CsNKL1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its expression, genetic organization, and biological effect on pathogen infection. CsNKL1 is composed of 135 residues and shares 33.1-56.5% overall sequence identities with other teleost NK-lysin. CsNKL1 possesses a Saposin B domain and six conserved cysteine residues that in mammals are known to form three intrachain disulfide bonds essential to antimicrobial activity. The genomic sequence of the ORF region of CsNKL1 is 1240 bp in length and, like human NK-lysin, contains five exons and four introns. Expression of CsNKL1 occurred in multiple tissues and was upregulated by bacterial and viral infection in a time dependent manner. When CsNKL1 was overexpressed in tongue sole, significant upregulation of interleukin-1 and chemokines was observed in spleen and head kidney. Following bacterial and viral infection, the pathogen loads in the tissues of CsNKL1-overexpressing fish were significantly lower than those in control fish. These results indicate that CsNKL1 possesses the novel capacities of immunomodulation and enhancing antimicrobial defense against pathogens of both bacterial and viral nature. (C) 2013 Elsevier Ltd. All rights reserved.; NK-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. Mammalian NK-lysin is known to possess antibacterial property and antitumor activity. Homologues of NK-lysin have been identified in several teleost species, but the natural function of fish NK-lysin remains essentially unknown. In this study, we identified a NK-lysin, CsNKL1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its expression, genetic organization, and biological effect on pathogen infection. CsNKL1 is composed of 135 residues and shares 33.1-56.5% overall sequence identities with other teleost NK-lysin. CsNKL1 possesses a Saposin B domain and six conserved cysteine residues that in mammals are known to form three intrachain disulfide bonds essential to antimicrobial activity. The genomic sequence of the ORF region of CsNKL1 is 1240 bp in length and, like human NK-lysin, contains five exons and four introns. Expression of CsNKL1 occurred in multiple tissues and was upregulated by bacterial and viral infection in a time dependent manner. When CsNKL1 was overexpressed in tongue sole, significant upregulation of interleukin-1 and chemokines was observed in spleen and head kidney. Following bacterial and viral infection, the pathogen loads in the tissues of CsNKL1-overexpressing fish were significantly lower than those in control fish. These results indicate that CsNKL1 possesses the novel capacities of immunomodulation and enhancing antimicrobial defense against pathogens of both bacterial and viral nature. (C) 2013 Elsevier Ltd. All rights reserved.
关键词	Nk-lysin Antimicrobial Peptide Cynoglossus Semilaevis Bacterial And Viral Infection
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2013.03.005
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000321091100005
WOS关键词	CYTOTOXIC T-CELLS ; CHANNEL CATFISH ; PEPTIDE NK-2 ; MOLECULAR-CLONING ; JAPANESE FLOUNDER ; GENOMIC STRUCTURE ; EFFECTOR PEPTIDE ; CANCER-CELLS ; CORE-REGION ; GENE
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：55[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16514
专题	实验海洋生物学重点实验室研究生部
通讯作者	Sun, L
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Min,Long, Hao,Sun, Li,et al. A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,40(3-4):258-265.
APA	Zhang, Min,Long, Hao,Sun, Li,&Sun, L.(2013).A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,40(3-4),258-265.
MLA	Zhang, Min,et al."A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 40.3-4(2013):258-265.

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