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A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens
Zhang, Min1; Long, Hao1,2; Sun, Li1; Sun, L
2013-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume40Issue:3-4Pages:258-265
SubtypeArticle
AbstractNK-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. Mammalian NK-lysin is known to possess antibacterial property and antitumor activity. Homologues of NK-lysin have been identified in several teleost species, but the natural function of fish NK-lysin remains essentially unknown. In this study, we identified a NK-lysin, CsNKL1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its expression, genetic organization, and biological effect on pathogen infection. CsNKL1 is composed of 135 residues and shares 33.1-56.5% overall sequence identities with other teleost NK-lysin. CsNKL1 possesses a Saposin B domain and six conserved cysteine residues that in mammals are known to form three intrachain disulfide bonds essential to antimicrobial activity. The genomic sequence of the ORF region of CsNKL1 is 1240 bp in length and, like human NK-lysin, contains five exons and four introns. Expression of CsNKL1 occurred in multiple tissues and was upregulated by bacterial and viral infection in a time dependent manner. When CsNKL1 was overexpressed in tongue sole, significant upregulation of interleukin-1 and chemokines was observed in spleen and head kidney. Following bacterial and viral infection, the pathogen loads in the tissues of CsNKL1-overexpressing fish were significantly lower than those in control fish. These results indicate that CsNKL1 possesses the novel capacities of immunomodulation and enhancing antimicrobial defense against pathogens of both bacterial and viral nature. (C) 2013 Elsevier Ltd. All rights reserved.; NK-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. Mammalian NK-lysin is known to possess antibacterial property and antitumor activity. Homologues of NK-lysin have been identified in several teleost species, but the natural function of fish NK-lysin remains essentially unknown. In this study, we identified a NK-lysin, CsNKL1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its expression, genetic organization, and biological effect on pathogen infection. CsNKL1 is composed of 135 residues and shares 33.1-56.5% overall sequence identities with other teleost NK-lysin. CsNKL1 possesses a Saposin B domain and six conserved cysteine residues that in mammals are known to form three intrachain disulfide bonds essential to antimicrobial activity. The genomic sequence of the ORF region of CsNKL1 is 1240 bp in length and, like human NK-lysin, contains five exons and four introns. Expression of CsNKL1 occurred in multiple tissues and was upregulated by bacterial and viral infection in a time dependent manner. When CsNKL1 was overexpressed in tongue sole, significant upregulation of interleukin-1 and chemokines was observed in spleen and head kidney. Following bacterial and viral infection, the pathogen loads in the tissues of CsNKL1-overexpressing fish were significantly lower than those in control fish. These results indicate that CsNKL1 possesses the novel capacities of immunomodulation and enhancing antimicrobial defense against pathogens of both bacterial and viral nature. (C) 2013 Elsevier Ltd. All rights reserved.
KeywordNk-lysin Antimicrobial Peptide Cynoglossus Semilaevis Bacterial And Viral Infection
Subject AreaImmunology ; Zoology
DOI10.1016/j.dci.2013.03.005
URL查看原文
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000321091100005
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/16514
Collection实验海洋生物学重点实验室
研究生部
Corresponding AuthorSun, L
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Min,Long, Hao,Sun, Li,et al. A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2013,40(3-4):258-265.
APA Zhang, Min,Long, Hao,Sun, Li,&Sun, L.(2013).A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,40(3-4),258-265.
MLA Zhang, Min,et al."A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 40.3-4(2013):258-265.
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