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| A new shrimp peritrophin-like gene from Exopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection | |
Wang, Liyan1,2; Li, Fuhua2; Wang, Bing2; Xiang, Jianhai2 ; Xiang, JH
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| 2013-09-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 35期号:3页码:840-846 |
| 文章类型 | Article |
| 摘要 | Peritrophin was first separated from insect peritrophic membrane (PM), and it played an important role in stimulating the digestion of food and protecting insects from invasion by microorganisms. In this study, a full-length cDNA of a new peritrophin-like protein (EcPT) was cloned from the ridgetail white shrimp Exopalaemon carinicauda, which was an excellent experimental animal for shrimp. The full length cDNA comprised 1235 bp including an 873 bp open reading frame encoding 291 amino acids. The deduced amino acid sequence contained a segment of signal peptide and three conserved chitin binding type 2 domains (ChtBD2) characterized by having a 6-cysteine motif. Tissue expression analysis revealed that EcPT was mainly expressed in stomach and gills, which were also the two main target tissues of WSSV infection. The transcription levels of EcPT in both stomach and gills were found to have significantly changed upon WSSV infection by real-time PCR. Silencing EcPT by dsRNA interference led to higher survival rate of shrimp against WSSV challenge, which suggested that EcPT might be involved in WSSV infection. (C) 2013 Elsevier Ltd. All rights reserved.; Peritrophin was first separated from insect peritrophic membrane (PM), and it played an important role in stimulating the digestion of food and protecting insects from invasion by microorganisms. In this study, a full-length cDNA of a new peritrophin-like protein (EcPT) was cloned from the ridgetail white shrimp Exopalaemon carinicauda, which was an excellent experimental animal for shrimp. The full length cDNA comprised 1235 bp including an 873 bp open reading frame encoding 291 amino acids. The deduced amino acid sequence contained a segment of signal peptide and three conserved chitin binding type 2 domains (ChtBD2) characterized by having a 6-cysteine motif. Tissue expression analysis revealed that EcPT was mainly expressed in stomach and gills, which were also the two main target tissues of WSSV infection. The transcription levels of EcPT in both stomach and gills were found to have significantly changed upon WSSV infection by real-time PCR. Silencing EcPT by dsRNA interference led to higher survival rate of shrimp against WSSV challenge, which suggested that EcPT might be involved in WSSV infection. (C) 2013 Elsevier Ltd. All rights reserved. |
| 关键词 | Peritrophin Chitin Binding Protein Wssv Ridgetail White Shrimp Exopalaemon Carinicauda |
| 学科领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2013.06.018 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000324511700026 |
| WOS关键词 | PENAEUS-MONODON ; MOLECULAR CHARACTERIZATION ; FENNEROPENAEUS-CHINENSIS ; BACULOVIRUS WSBV ; LITOPENAEUS-VANNAMEI ; PROTEIN ; MEMBRANE ; MATRIX ; DISRUPTS ; IDENTIFICATION |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16512 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xiang, JH |
| 作者单位 | 1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Liyan,Li, Fuhua,Wang, Bing,et al. A new shrimp peritrophin-like gene from Exopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):840-846. |
| APA | Wang, Liyan,Li, Fuhua,Wang, Bing,Xiang, Jianhai,&Xiang, JH.(2013).A new shrimp peritrophin-like gene from Exopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection.FISH & SHELLFISH IMMUNOLOGY,35(3),840-846. |
| MLA | Wang, Liyan,et al."A new shrimp peritrophin-like gene from Exopalaemon carinicauda involved in white spot syndrome virus (WSSV) infection".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):840-846. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A new shrimp peritro（1743KB） | 限制开放 | CC BY-NC-SA | 浏览 | |||
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