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Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation
Li, Yuanxiang1,2,3; Huang, Aiyou1,2; Gu, Wenhui1,2; Wu, Songcui1,2; Xie, Xiujun1,2; Wang, Guangce1,2
2020-01-02
Source PublicationEUROPEAN JOURNAL OF PHYCOLOGY
ISSN0967-0262
Volume55Issue:1Pages:64-75
Corresponding AuthorWang, Guangce(gcwang@qdio.ac.cn)
AbstractMicroalgae are capable of accumulating biomass and high value-added products in mixotrophic culture. Carbon sources and light intensity have significant impacts on mixotrophic algal growth and metabolites accumulation. To investigate the underlying mechanism of interaction between metabolism of inorganic carbon and organic carbon, growth of Nannochloropsis oceanica with two different carbon sources (organic sodium acetate and inorganic sodium bicarbonate) and under three different light conditions (low, medium and high) was examined. A combination of sodium acetate and sodium bicarbonate significantly enhanced algal growth with higher acetate assimilation, when compared with single carbon sources. With increasing light intensity from low to high, the algal growth rates decreased in both autotrophic and mixotrophic cultures. Proteome and C-13 metabolic flux results showed that acetate utilization via the glyoxylate (GOX) cycle was enhanced, and both the tricarboxylic acid (TCA) cycle and putative C-4 pathway were stimulated in the presence of sodium acetate and sodium bicarbonate. These data suggest that sodium bicarbonate could facilitate acetate assimilation and enhance the putative C-4 pathway, GOX cycle and TCA cycle in N. oceanica under mixotrophic culture conditions.
KeywordC-13 metabolic analysis light mixotrophic Nannochloropsis sodium acetate sodium bicarbonate
DOI10.1080/09670262.2019.1660808
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41876158] ; National Natural Science Foundation of China[41806171] ; National Key R&D Program of China[2018YFD0901500] ; Haikou Marine Economic Demonstration City Industry Chain Collaborative Innovation Project[HHCL201811] ; Tianjin Marine Economic Demonstration[BHSF2017-21]
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000520076900007
PublisherTAYLOR & FRANCIS LTD
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165077
Collection中国科学院海洋研究所
Corresponding AuthorWang, Guangce
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Yuanxiang,Huang, Aiyou,Gu, Wenhui,et al. Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2020,55(1):64-75.
APA Li, Yuanxiang,Huang, Aiyou,Gu, Wenhui,Wu, Songcui,Xie, Xiujun,&Wang, Guangce.(2020).Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation.EUROPEAN JOURNAL OF PHYCOLOGY,55(1),64-75.
MLA Li, Yuanxiang,et al."Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation".EUROPEAN JOURNAL OF PHYCOLOGY 55.1(2020):64-75.
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