Omithinimicrobium tianjinense sp nov., isolated from a recirculating aquaculture system

doi:10.1099/ijs.0.052514-0

	Omithinimicrobium tianjinense sp nov., isolated from a recirculating aquaculture system
	Liu, Liang-Zi 1,2; Liu, Ying1 ; Chen, Zhu 3; Liu, Hong-Can 1; Zhou, Yu-Guang 1; Liu, Zhi-Pei 1; Liu, ZP
	2013-12-01
发表期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN	1466-5026
卷号	63 页码:4489-4494
文章类型	Article
摘要	A Gram-positive, strictly aerobic and heterotrophic, non-spore-forming actinobacterium (strain B2(T)) isolated from a recirculating aquaculture system was studied for its taxonomic position. Strain B2(T) formed a rudimentary substrate-mycelium that fragmented into short rod-shaped to coccoid cells (0.5 mu mx0.5-2.2 mu m or 0.5-1.0 j.tm in diameter). Colonies were yellow, smooth, circular and 1.5-2.0 mm in diameter after incubation on TSA for 3 days at 30 degrees C. Strain B2(T) grew at 20-40 degrees C (optimal, 30 degrees C) and pH 5.5-9.5 (optimal, 6.5-7.0) and in the presence of 0-9 % (w/v) NaCl (optimal, 1%). The predominant menaquinone of strain B2(T) was MK-8(F14). The cell-wall peptidoglycan of strain B2(T) contained the amino acids ornithine, glutamic acid, alanine, glycine and aspartic acid. The major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C16,0, iso-C16:0 and summed feature 9. Its DNA G + C content was 68.3 mol% (T-m). Analysis of 16S rRNA gene sequences indicated that strain B2(T) was related phylogenetically to members of the genus Ornithinimicrobium with highest similarity (96.6 %) to Omithinimicrobium kibberense DSM 17687(T), followed by Omithinimicrobium humiphilum DSM 12362(T) (96.3 %), Omithinimicrobium pekingense LW6(T) (96.2%) and Omithinimicrobium murale 01-Gi-040(T) (94.8%). On basis of phenotypic, chemotaxonomic and phylogenetic data, it was concluded that strain B2(T) represents a novel species of the genus Omithinimicrobium, for which the name Omithinimicrobium tianjinense sp. nov. is proposed. The type strain is B2(T) (=CGMCC 1.12160(T)=JCM 18464(T)).; A Gram-positive, strictly aerobic and heterotrophic, non-spore-forming actinobacterium (strain B2(T)) isolated from a recirculating aquaculture system was studied for its taxonomic position. Strain B2(T) formed a rudimentary substrate-mycelium that fragmented into short rod-shaped to coccoid cells (0.5 mu mx0.5-2.2 mu m or 0.5-1.0 j.tm in diameter). Colonies were yellow, smooth, circular and 1.5-2.0 mm in diameter after incubation on TSA for 3 days at 30 degrees C. Strain B2(T) grew at 20-40 degrees C (optimal, 30 degrees C) and pH 5.5-9.5 (optimal, 6.5-7.0) and in the presence of 0-9 % (w/v) NaCl (optimal, 1%). The predominant menaquinone of strain B2(T) was MK-8(F14). The cell-wall peptidoglycan of strain B2(T) contained the amino acids ornithine, glutamic acid, alanine, glycine and aspartic acid. The major polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were iso-C16,0, iso-C16:0 and summed feature 9. Its DNA G + C content was 68.3 mol% (T-m). Analysis of 16S rRNA gene sequences indicated that strain B2(T) was related phylogenetically to members of the genus Ornithinimicrobium with highest similarity (96.6 %) to Omithinimicrobium kibberense DSM 17687(T), followed by Omithinimicrobium humiphilum DSM 12362(T) (96.3 %), Omithinimicrobium pekingense LW6(T) (96.2%) and Omithinimicrobium murale 01-Gi-040(T) (94.8%). On basis of phenotypic, chemotaxonomic and phylogenetic data, it was concluded that strain B2(T) represents a novel species of the genus Omithinimicrobium, for which the name Omithinimicrobium tianjinense sp. nov. is proposed. The type strain is B2(T) (=CGMCC 1.12160(T)=JCM 18464(T)).
学科领域	Microbiology
DOI	10.1099/ijs.0.052514-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000330922400018
WOS关键词	SEQUENCE ALIGNMENT ; L-ORNITHINE ; GEN. NOV. ; ACTINOMYCETE ; PEPTIDOGLYCAN ; SERINICOCCUS ; SEDIMENT ; DNA
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16505
专题	海洋生态与环境科学重点实验室海洋生物技术研发中心
通讯作者	Liu, ZP
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Liu, Liang-Zi,Liu, Ying,Chen, Zhu,et al. Omithinimicrobium tianjinense sp nov., isolated from a recirculating aquaculture system[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2013,63:4489-4494.
APA	Liu, Liang-Zi.,Liu, Ying.,Chen, Zhu.,Liu, Hong-Can.,Zhou, Yu-Guang.,...&Liu, ZP.(2013).Omithinimicrobium tianjinense sp nov., isolated from a recirculating aquaculture system.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,63,4489-4494.
MLA	Liu, Liang-Zi,et al."Omithinimicrobium tianjinense sp nov., isolated from a recirculating aquaculture system".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 63(2013):4489-4494.

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