Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Effects of environmental stress (sex steroids and heat) during sex differentiation in Japanese flounder (Paralichthys olivaceus): insight from germ cell proliferation and gsdf-amh-cyp19a1a expression | |
Yang, Yang1,2,3,4,6 ; Liu, Qinghua2,3,6; Xiao, Yongshuang2,3,6; Xu, Shihong2,3,6; Wang, Xueying2,3,6; Yang, Jingkun2,3,6; Song, Zongcheng5; You, Feng2,3,6; Li, Jun2,3,6
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| 2020-01-15 | |
| Source Publication | AQUACULTURE
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| ISSN | 0044-8486 |
| Volume | 515Pages:8 |
| Corresponding Author | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| Abstract | Marine environments have been underwent many changes and threats due to human activities and climate variation. The available information regarding the effects of environmental changes on reproduction in marine fishes is limited. In this study, we investigated gonadal development with special attention on sex differentiation in genetically female Japanese flounders (Paralichthys olivaceus) larvae exposed to 17 beta-Estradiol (E2), 17 alpha-Methyltestosterone (MT) and heat treatment (27.5 +/- 0.5 degrees C) respectively from 30 to 120 dph. Results indicated that E2 obviously accelerated ovarian differentiation and inhibited germ cell proliferation, suggesting the exogenous estrogen was toxic to reproduction of genetically female fishes. The exogenous androgen and high temperature induced masculinization of genetically female flounders, accompanied by inhibiting the proliferation of germ cells. Meanwhile, the expression patterns of sex-related genes illustrated that the mechanisms of sex-reversal induced by high temperature and exogenous androgen may be different, with respectively inducing the high expression level of gsdf and amh, finally resulting in decreasing of cyp19a1a expression and masculinization of XX flounders. These results would be useful to assess and understand the influence of aquatic environmental factors on reproduction in teleost. |
| Keyword | Gonadal development Japanese flounder High temperature Sex steroids gsdf/amh/cyp19a1a |
| DOI | 10.1016/j.aquaculture.2019.734536 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program[2018YFD0901204] ; National Key Research and Development Program[2018YFD0901205] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31372514] ; China Agriculture Research System[CARS-47] ; STS project[KFZD-SW-106] ; Shandong Province Key Research and Invention Program[2017CXGC010K] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000496787000011 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/165056 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Liu, Qinghua; Li, Jun |
| Affiliation | 1.Ningbo Univ, Coll Marine Sci, Ningbo 315832, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yang, Yang,Liu, Qinghua,Xiao, Yongshuang,et al. Effects of environmental stress (sex steroids and heat) during sex differentiation in Japanese flounder (Paralichthys olivaceus): insight from germ cell proliferation and gsdf-amh-cyp19a1a expression[J]. AQUACULTURE,2020,515:8. |
| APA | Yang, Yang.,Liu, Qinghua.,Xiao, Yongshuang.,Xu, Shihong.,Wang, Xueying.,...&Li, Jun.(2020).Effects of environmental stress (sex steroids and heat) during sex differentiation in Japanese flounder (Paralichthys olivaceus): insight from germ cell proliferation and gsdf-amh-cyp19a1a expression.AQUACULTURE,515,8. |
| MLA | Yang, Yang,et al."Effects of environmental stress (sex steroids and heat) during sex differentiation in Japanese flounder (Paralichthys olivaceus): insight from germ cell proliferation and gsdf-amh-cyp19a1a expression".AQUACULTURE 515(2020):8. |
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| Effects of environme（4237KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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