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Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates
Deng, Yunyan1,2,3; Hu, Zhangxi1,2,3; Chai, Zhaoyang1,2; Tang, Ying Zhong1,2,3
2019-10-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume31Issue:5Pages:2969-2979
Corresponding AuthorTang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
AbstractProliferating cell nuclear antigen (PCNA), a co-factor of DNA polymerases delta and epsilon, is associated with active cell proliferation, particularly with the S phase of the eukaryotic cell cycle, and the expression of this gene has been therefore proposed as a molecular marker for estimating phytoplankton growth rate and even population dynamics. In this study, the full-length cDNA sequences of PCNA for nine dinoflagellate species (ten strains) were obtained, analyzed, and further characterized with the expression in response to alterations of growth stage and life cycle. All the ten genes have a 777 or 780 bp ORF and encode a protein of 258 or 259 amino acids, similar to PCNAs from vertebrates and plants. The deduced amino acid sequences include conservative motif characteristic to the PCNA gene family, implying conserved functions of PCNA among organisms of different taxa. Alignment analysis in combination with other sequences available in NCBI database exhibited high amino acid similarities (79.2-96.9%) among PCNAs from 17 dinoflagellate species. Phylogenetic tree derived from 37 PCNA sequences of dinoflagellates was consistent with the taxonomic affiliation of these taxa. Then, we used the cosmopolitan, toxic, and resting cyst-producing dinoflagellate Scrippsiella trochoidea as a representative of HAB-forming dinoflagellates to investigate PCNA (StPCNA) expression at different stages of growth and life cycle and the results of real-time quantitative PCR revealed clearly a stage-dependent pattern in the transcription of StPCNA. The transcripts dramatically reduced from an exponential to a stationary growth stage and then further significantly decreased from a stationary stage to dormant stage (resting cysts), a pattern in accordance with that previously reported from the dinoflagellates Alexandrium catenella and Prorocentrum donghaiense. In terms of its minimal implication, this work enhances the previous perception about the function and possible application of PCNA gene, and more importantly, our results indicated that PCNA is not silent during the dormant stage of dinoflagellates.
KeywordDinoflagellate Harmful algal blooms (HABs) Proliferating cell nuclear antigen (PCNA) Resting cyst Scrippsiella trochoidea
DOI10.1007/s10811-019-01809-6
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[41476142] ; National Science Foundation of China[41506143] ; National Science Foundation of China[61533011] ; National Science Foundation of China[U1301235] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000493895800019
PublisherSPRINGER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164947
Collection海洋生态与环境科学重点实验室
Corresponding AuthorTang, Ying Zhong
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,et al. Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(5):2969-2979.
APA Deng, Yunyan,Hu, Zhangxi,Chai, Zhaoyang,&Tang, Ying Zhong.(2019).Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates.JOURNAL OF APPLIED PHYCOLOGY,31(5),2969-2979.
MLA Deng, Yunyan,et al."Cloning and comparative studies of proliferating cell nuclear antigen (PCNA) genes for nine dinoflagellates".JOURNAL OF APPLIED PHYCOLOGY 31.5(2019):2969-2979.
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