Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions

doi:10.1093/plankt/fbs080

	Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions
	Liu, Yun 1,2; Song, Xiuxian1 ; Cao, Xihua1 ; Yu, Zhiming1 ; Song, XX
	2013
发表期刊	JOURNAL OF PLANKTON RESEARCH
ISSN	0142-7873
卷号	35 期号:1 页码:165-176
文章类型	Article
摘要	The photosynthetic capacity of phytoplankton is frequently limited by the availability of nutrients. To explore the mechanism of the effect of nutrient availability on photosynthesis, batch cultures of a marine diatom Skeletonema costatum (Greville) Cleve were carried out under different nitrate (N: 0-220.75 mu M) and phosphate (P: 0-9.075 mu M) concentrations. Changes of photosynthetic characters, including the photosynthetic rate (measured by O-2 evolution) and the rbcL mRNA content (encoding the large subunit of the Calvin cycle enzyme, RuBisCO), were both studied. Within the range tested, both the photosynthetic rate and rbcL transcript levels correlated significantly with N and P concentrations in the medium. Additionally, the photosynthetic rates and rbcL transcript levels were both growth dependent. Significant correlations were also found between the abundance of rbcL mRNA and the photosynthetic rate (R-2 = 0.800), rbcL and growth rate (R-2 = 0.855), the photosynthetic rate and growth rate (R-2 = 0.815). These results are consistent with N and P nutrition-regulating photosynthesis from the transcriptional levels of rbcL. The rbcL transcript level has the potential to be a good marker of algal growth and primary productivity.; The photosynthetic capacity of phytoplankton is frequently limited by the availability of nutrients. To explore the mechanism of the effect of nutrient availability on photosynthesis, batch cultures of a marine diatom Skeletonema costatum (Greville) Cleve were carried out under different nitrate (N: 0-220.75 mu M) and phosphate (P: 0-9.075 mu M) concentrations. Changes of photosynthetic characters, including the photosynthetic rate (measured by O-2 evolution) and the rbcL mRNA content (encoding the large subunit of the Calvin cycle enzyme, RuBisCO), were both studied. Within the range tested, both the photosynthetic rate and rbcL transcript levels correlated significantly with N and P concentrations in the medium. Additionally, the photosynthetic rates and rbcL transcript levels were both growth dependent. Significant correlations were also found between the abundance of rbcL mRNA and the photosynthetic rate (R-2 = 0.800), rbcL and growth rate (R-2 = 0.855), the photosynthetic rate and growth rate (R-2 = 0.815). These results are consistent with N and P nutrition-regulating photosynthesis from the transcriptional levels of rbcL. The rbcL transcript level has the potential to be a good marker of algal growth and primary productivity.
关键词	Photosynthetic Rate Rbcl Transcript Levels Nutrient Concentrations Quantitative Real-time Pcr Skeletonema Costatum
学科领域	Marine & Freshwater Biology ; Oceanography
DOI	10.1093/plankt/fbs080
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Marine & Freshwater Biology ; Oceanography
WOS记录号	WOS:000312883300015
WOS关键词	RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OXYGENASE ; ISOCHRYSIS-GALBANA HAPTOPHYCEAE ; ULVA-RIGIDA CHLOROPHYTA ; NITROGEN LIMITATION ; PHOSPHORUS LIMITATION ; INORGANIC-PHOSPHATE ; GENE-EXPRESSION ; BIOCHEMICAL-COMPOSITION ; CHLOROPLAST PROTEINS ; RIVER ESTUARY
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16489
专题	海洋生态与环境科学重点实验室
通讯作者	Song, XX
作者单位	1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Yun,Song, Xiuxian,Cao, Xihua,et al. Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions[J]. JOURNAL OF PLANKTON RESEARCH,2013,35(1):165-176.
APA	Liu, Yun,Song, Xiuxian,Cao, Xihua,Yu, Zhiming,&Song, XX.(2013).Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions.JOURNAL OF PLANKTON RESEARCH,35(1),165-176.
MLA	Liu, Yun,et al."Responses of photosynthetic characters of Skeletonema costatum to different nutrient conditions".JOURNAL OF PLANKTON RESEARCH 35.1(2013):165-176.

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