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Transcriptome profiling of the meristem tissue of Saccharina japonica (Phaeophyceae, Laminariales) under severe stress of copper
Zhang, Yurong1,2; Wang, Xuemei3,4,5,6; Shan, Tifeng3,4,5; Pang, Shaojun3,4,5; Xu, Nianjun1
2019-10-01
Source PublicationMARINE GENOMICS
ISSN1874-7787
Volume47Pages:10
Corresponding AuthorShan, Tifeng(shantifeng@qdio.ac.cn) ; Pang, Shaojun(sjpang@qdio.ac.cn)
AbstractCopper (Cu) is an essential metal involved in many physiological processes of living organisms. However, beyond a certain threshold, Cu can become highly toxic. For instance, in the summer sporeling production of the economic kelp Saccharina japonica, the excess Cu accidently released from the low-quality alloys of the refrigerating machine was deadly to the seedlings and led to the failure of hatchery operations. However, the molecular basis underlying high toxicity of Cu remains unclear. In this study, juvenile sporophytes were cultured in seawater containing different concentrations of Cu2+ (10, 100, and 200 mu g L-1). Bleaching was observed in the meristem of individuals in the 100 and 200 mu g L-1 treatment groups on the third day, indicating that Cu has caused severe harm at these concentrations. RNA-Seq was used to profile transcriptomic changes under different Cu2+ concentrations. Compared with the control, the number of differentially expressed genes (DEGs) was 11,350 (4944 up- and 6406 down-regulated) in the 200 mu g L-1 treatment group and 2868 (1075 up- and 1793 down-regulated) in the 100 mu g L-1 treatment group, whereas much fewer DEGs were detected in the 10 mu g L-1 treatment group. Genes coding for glutathione-S-transferase and vanadium-dependent bromoperoxidase and iodoperoxidase were found to be remarkably regulated, especially in the 200 mu g L-1 treatment group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that only down-regulated DEGs were enriched. There were 45 enriched GO terms and four enriched KEGG pathways common to the 100 and 200 mu g L-1 treatment groups, which were associated with diverse essential biological processes such as photosynthesis, protein synthesis, redox activity, and metabolism and biosynthesis of functional biomolecules, among others. Suppression of these biological processes at the transcriptional level likely contributes to the observed high toxicity of Cu2+ in S. japonica.
KeywordKelp Cu Illumina sequencing Seaweed Heavy metal Toxicity
DOI10.1016/j.margen.2019.03.006
Indexed BySCI
Language英语
Funding ProjectState's Key Project of Research and Development Plan[2016YFC1402507] ; Ministry of Science and Technology of China ; Taishan Scholar Program of Shandong Province of China ; Project of Zhejiang Province of China[LGF19D060002] ; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences
WOS Research AreaGenetics & Heredity ; Marine & Freshwater Biology
WOS SubjectGenetics & Heredity ; Marine & Freshwater Biology
WOS IDWOS:000493212700001
PublisherELSEVIER
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164893
Collection实验海洋生物学重点实验室
Corresponding AuthorShan, Tifeng; Pang, Shaojun
Affiliation1.Ningbo Univ, Sch Marine Sci, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Zhejiang, Peoples R China
2.Marine Fishery Inst Zhejiang Prov, Key Lab Maricuirure & Enhancement Zhejiang Prov, Zhoushan 316100, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Yurong,Wang, Xuemei,Shan, Tifeng,et al. Transcriptome profiling of the meristem tissue of Saccharina japonica (Phaeophyceae, Laminariales) under severe stress of copper[J]. MARINE GENOMICS,2019,47:10.
APA Zhang, Yurong,Wang, Xuemei,Shan, Tifeng,Pang, Shaojun,&Xu, Nianjun.(2019).Transcriptome profiling of the meristem tissue of Saccharina japonica (Phaeophyceae, Laminariales) under severe stress of copper.MARINE GENOMICS,47,10.
MLA Zhang, Yurong,et al."Transcriptome profiling of the meristem tissue of Saccharina japonica (Phaeophyceae, Laminariales) under severe stress of copper".MARINE GENOMICS 47(2019):10.
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