IOCAS-IR
Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.
Zhou, Li; Wang, Xueying; Liu, Qinghua; Xu, Shihong; Zhao, Haixia; Han, Mingming; Wang, Yunong; Song, Zongcheng; Li, Jun
2019-10-01
Source PublicationMarine Biotechnology (New York Springer)
ISSN1436-2228
Volume21Issue:5Pages:671-682
AbstractPrimordial germ cells (PGCs) as the precursors of germ cells are responsible for transmitting genetic information to the next generation. Visualization of teleost PGCs in vivo is essential to research the origination and development of germ cells and facilitate further manipulation on PGCs isolation, cryopreservation, and surrogate breeding. In this study, artificially synthesized mRNAs constructed by fusing fluorescent protein coding region to the 3' untranslated region (3'UTR) of nanos3 or vasa (mCherry-Smnanos3 3'UTR or mCherry-Smvasa 3'UTR mRNA) were injected into turbot (Scophthalmus maximus) fertilized eggs for tracing PGCs. The results demonstrated that the fluorescent PGCs differentiated from somatic cells and aligned on both sides of the trunk at the early segmentation period, then migrated and located at the dorsal part of the gut where the gonad would form. In the same way, we also found that the zebrafish (Danio rerio) vasa 3'UTR could trace turbot PGCs, while the vasa 3'UTR s of marine medaka (Oryzias melastigma) and red seabream (Pagrus major) failed, although they could label the marine medaka PGCs. In addition, through comparative analysis, we discovered that some potential sequence elements in the3 'UTRs of nanos3 and vasa, such as GCACs, 62-bp U-rich regions and nucleotide 187-218 regions might be involved in PGCs stabilization. The results of this study provided an efficient, rapid, and specific non-transgenic approach for visualizing PGCs of economical marine fish in vivo.
Indexed BySCI
Language英语
WOS Research AreaZoology ; Reproductive Biology ; Developmental Biology
WOS SubjectZOOLOGY ; REPRODUCTIVE BIOLOGY ; DEVELOPMENTAL BIOLOGY
WOS IDWOS:ZOOR15512088599
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164881
Collection中国科学院海洋研究所
AffiliationChinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao, Shandong, 266071, China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhou, Li,Wang, Xueying,Liu, Qinghua,et al. Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.[J]. Marine Biotechnology (New York Springer),2019,21(5):671-682.
APA Zhou, Li.,Wang, Xueying.,Liu, Qinghua.,Xu, Shihong.,Zhao, Haixia.,...&Li, Jun.(2019).Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene..Marine Biotechnology (New York Springer),21(5),671-682.
MLA Zhou, Li,et al."Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.".Marine Biotechnology (New York Springer) 21.5(2019):671-682.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhou, Li]'s Articles
[Wang, Xueying]'s Articles
[Liu, Qinghua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhou, Li]'s Articles
[Wang, Xueying]'s Articles
[Liu, Qinghua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhou, Li]'s Articles
[Wang, Xueying]'s Articles
[Liu, Qinghua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.