Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae) | |
Li, Yuanxiang1,2,3; Gu, Wenhui1,2; Huang, Aiyou1,2; Xie, Xiujun1,2; Wu, Songcui1,2 ; Wang, Guangce1,2
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| 2019-10-01 | |
| 发表期刊 | PHYCOLOGICAL RESEARCH
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| ISSN | 1322-0829 |
| 卷号 | 67期号:4页码:291-302 |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 摘要 | The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina. |
| 关键词 | carotenoid light treatment microalgae photosynthesis photosynthetic activity |
| DOI | 10.1111/pre.12379 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41506188] ; China Nantong Municipal Applied Basic Research Program[MS12017025-2] ; Tianjin Demonstration Project for Innovative Development of Marine Economy[BHSF2017-21] |
| WOS研究方向 | Marine & Freshwater Biology |
| WOS类目 | Marine & Freshwater Biology |
| WOS记录号 | WOS:000491488900005 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/164869 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Yuanxiang,Gu, Wenhui,Huang, Aiyou,et al. Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)[J]. PHYCOLOGICAL RESEARCH,2019,67(4):291-302. |
| APA | Li, Yuanxiang,Gu, Wenhui,Huang, Aiyou,Xie, Xiujun,Wu, Songcui,&Wang, Guangce.(2019).Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae).PHYCOLOGICAL RESEARCH,67(4),291-302. |
| MLA | Li, Yuanxiang,et al."Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)".PHYCOLOGICAL RESEARCH 67.4(2019):291-302. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Transcriptome analys（778KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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