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A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus
Wang, Yan1,3; Wang, Baojie1; Shao, Xuqing6; Liu, Mei1; Jiang, Keyong1; Wang, Mengqiang1,4; Wang, Lei1,2,5
2019-11-14
Source PublicationMOLECULAR GENETICS AND GENOMICS
ISSN1617-4615
Pages13
Corresponding AuthorWang, Mengqiang(wangmengqiang@qdio.ac.cn) ; Wang, Lei(wanglei@qdio.ac.cn)
AbstractThe red claw crayfish (Cherax quadricarinatus) is an emerging and important commercial species in several countries, and is also a potential biological model in crustacean biology. However, its molecular embryonic development mechanism remains largely unknown because of a lack of genomic resources and systematic research. A comprehensive and integrated transcriptomic analysis is necessary to reveal the cell biological function, gene expression profiles, and embryo patterning that occur during embryogenesis. In the present study, transcriptomic profiles of C. quadricarinatus embryos during three developmental stages were investigated by high-throughput Illumina sequencing technology, and the genes related to development were further analyzed. In total, 49,436 unigenes were assembled and clustered, in which 13,727 were annotated in the Nonredundant database, 5087 were classified based on Gene Ontology annotations, and 2735 were associated with 189 Kyoto Encyclopedia of Genes and Genomes pathways. Furthermore, gene expression differences among the embryos stages were analyzed, and 6658 differentially expressed genes (DEGs) were identified. In total, 3300, 5211, and 1262 DEGs were identified between the eye pigments forming stage (EP) and prepare-hatching stage (PH), EP and larvae (L), as well as PH and L; meanwhile, 1595, 2540 and 680 DEGs were annotated, respectively. The fundamental developmental genes related to apoptosis, neurogenesis, and segmentation, as well as signaling pathways related to Hedgehog, MAPK, Wnt, TGF-beta and Notch, showed higher expression during the EP stage than in other two stages, indicating that the EP stage has more active biological processes than the latter stages. This transcriptome studies gene expression at different stages of embryonic development and the datasets provide a basis for understanding crustacean developmental biology and guiding seedling production.
KeywordCherax quadricarinatus Embryonic development Transcriptome
DOI10.1007/s00438-019-01621-4
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[U1706209] ; Marine S&T Fund of Shandong Province for National Laboratory for Marine Science and Technology[2018SDKJ0502-2]
WOS Research AreaBiochemistry & Molecular Biology ; Genetics & Heredity
WOS SubjectBiochemistry & Molecular Biology ; Genetics & Heredity
WOS IDWOS:000496200800001
PublisherSPRINGER HEIDELBERG
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164866
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Mengqiang; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Shandong, Peoples R China
5.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China
6.Shandong Cigna Detect Technol Co Ltd, Qingdao 266101, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Yan,Wang, Baojie,Shao, Xuqing,et al. A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus[J]. MOLECULAR GENETICS AND GENOMICS,2019:13.
APA Wang, Yan.,Wang, Baojie.,Shao, Xuqing.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus.MOLECULAR GENETICS AND GENOMICS,13.
MLA Wang, Yan,et al."A comparative transcriptomic analysis in late embryogenesis of the red claw crayfish Cherax quadricarinatus".MOLECULAR GENETICS AND GENOMICS (2019):13.
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