Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Evidence for Production of Sexual Resting Cysts by the Toxic Dinoflagellate Karenia mikimotoi in Clonal Cultures and Marine Sediments | |
Liu, Yuyang1,2,3; Hu, Zhangxi1,2,4 ; Deng, Yunyan1,2,4; Tang, Ying Zhong1,2,4
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| 2019-11-04 | |
| Source Publication | JOURNAL OF PHYCOLOGY
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| ISSN | 0022-3646 |
| Pages | 14 |
| Corresponding Author | Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) |
| Abstract | The toxic dinoflagellate Karenia mikimotoi has been well-known for causing large-scale and dense harmful algal blooms (HABs) in coastal waters worldwide and serious economic loss in aquaculture and fisheries and other adverse effects on marine ecosystems. Whether K. mikimotoi forms resting cysts has been a puzzling issue regarding to the mechanisms of bloom initiation and geographic expansion of this species. We provide morphological and molecular confirmation of sexually produced thin-walled resting cysts by K. mikimotoi based on observations of laboratory cultures and their direct detection in marine sediments. Light and scanning electron microscopy evidences for sexual reproduction include attraction and pairing of gametes, gamete fusion, formation of planozygote and thin-walled cyst, and the documentation of the thin-walled cyst germination processes. Evidence for cysts in marine sediments was in three aspects: positive PCR detection of cysts using species-specific primers in the DNA extracted from whole sediments; fluorescence in situ hybridization detection of cysts using FISH probes; and single-cell PCR sequencing for cysts positively labeled with FISH probes. The existence of sexually produced, thin-walled resting cysts by K. mikimotoi provides a possible mechanism accounting for the initiation of annually recurring blooms at certain regions and global expansion of the species during the past decades. |
| Keyword | fluorescence in situ hybridization harmful algal blooms (HABs) Karenia mikimotoi life cycle (history) resting cyst |
| DOI | 10.1111/jpy.12925 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Plant Sciences ; Marine & Freshwater Biology |
| WOS Subject | Plant Sciences ; Marine & Freshwater Biology |
| WOS ID | WOS:000493785200001 |
| Publisher | WILEY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/164857 |
| Collection | 海洋生态与环境科学重点实验室 |
| Corresponding Author | Tang, Ying Zhong |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu, Yuyang,Hu, Zhangxi,Deng, Yunyan,et al. Evidence for Production of Sexual Resting Cysts by the Toxic Dinoflagellate Karenia mikimotoi in Clonal Cultures and Marine Sediments[J]. JOURNAL OF PHYCOLOGY,2019:14. |
| APA | Liu, Yuyang,Hu, Zhangxi,Deng, Yunyan,&Tang, Ying Zhong.(2019).Evidence for Production of Sexual Resting Cysts by the Toxic Dinoflagellate Karenia mikimotoi in Clonal Cultures and Marine Sediments.JOURNAL OF PHYCOLOGY,14. |
| MLA | Liu, Yuyang,et al."Evidence for Production of Sexual Resting Cysts by the Toxic Dinoflagellate Karenia mikimotoi in Clonal Cultures and Marine Sediments".JOURNAL OF PHYCOLOGY (2019):14. |
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