Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)

doi:10.1111/j.1365-2109.2009.02466.x

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	Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)
	Liu, Qing Hua 1; Chen, Ya Kun 1,2; Xiao, Zhi Zhong 1; Li, Jun 1; Xu, Shi Hong 1; Shi, Xue Hui 3; Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
	2010-08-16
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
卷号	41 期号:9 页码:e89-e95
文章类型	Article
摘要	This study examined the effects of storage time and cryoprotectant concentrations on the post-thaw sperm of red seabream, Pagrus major. Sperm treated with 12%, 15%, 18% and 21% DMSO were cryopreserved for 10, 30, 60 and 360 days, and fertilization and hatching rates were analysed. For all groups, there were no differences in the fertilization rates and hatching rates between sperm cryopreserved for < 60 days and fresh sperm (98.8 +/- 0.8%, 96.4 +/- 1.3%). However, for sperm cryopreserved for 360 days, both fertilization rates (88.6 +/- 3.0% to 7.0 +/- 1.9%) and hatching rates (79.4 +/- 7.2% to 3.3 +/- 0.8%) decreased drastically. Furthermore, the cryoprotectant concentrations affected sperm quality significantly (P < 0.05). When cryopreserved for 360 days, sperm treated with 15% DMSO obtained the best results compared with other concentrations. We suggest that 15% DMSO may be an effective cryoprotectant for long-term sperm cryopreservation of red seabream.; This study examined the effects of storage time and cryoprotectant concentrations on the post-thaw sperm of red seabream, Pagrus major. Sperm treated with 12%, 15%, 18% and 21% DMSO were cryopreserved for 10, 30, 60 and 360 days, and fertilization and hatching rates were analysed. For all groups, there were no differences in the fertilization rates and hatching rates between sperm cryopreserved for < 60 days and fresh sperm (98.8 +/- 0.8%, 96.4 +/- 1.3%). However, for sperm cryopreserved for 360 days, both fertilization rates (88.6 +/- 3.0% to 7.0 +/- 1.9%) and hatching rates (79.4 +/- 7.2% to 3.3 +/- 0.8%) decreased drastically. Furthermore, the cryoprotectant concentrations affected sperm quality significantly (P < 0.05). When cryopreserved for 360 days, sperm treated with 15% DMSO obtained the best results compared with other concentrations. We suggest that 15% DMSO may be an effective cryoprotectant for long-term sperm cryopreservation of red seabream.
关键词	Red Seabream Sperm Cryopreservation Storage Time Cryoprotectant
学科领域	Fisheries
DOI	10.1111/j.1365-2109.2009.02466.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000281059700010
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1647
专题	实验海洋生物学重点实验室
通讯作者	Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Coll Earth Sci, Grad Sch, Beijing, Peoples R China 3.QingdaoAgr Univ, Org Dept, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Qing Hua,Chen, Ya Kun,Xiao, Zhi Zhong,et al. Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)[J]. AQUACULTURE RESEARCH,2010,41(9):e89-e95.
APA	Liu, Qing Hua.,Chen, Ya Kun.,Xiao, Zhi Zhong.,Li, Jun.,Xu, Shi Hong.,...&Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China.(2010).Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843).AQUACULTURE RESEARCH,41(9),e89-e95.
MLA	Liu, Qing Hua,et al."Effect of storage time and cryoprotectant concentrations on the fertilization rate and hatching rate of cryopreserved sperm in red seabream (Pagrus major Temminck & Schlegel, 1843)".AQUACULTURE RESEARCH 41.9(2010):e89-e95.

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