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	Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus
	Sun, L. N.1,2; Yang, H. S.1; Chen, M. Y.3; Xu, D. X.1,2; Yang, HS
	2013
发表期刊	GENETICS AND MOLECULAR RESEARCH
ISSN	1676-5680
卷号	12期号:4页码:5321-5334
文章类型	Article
摘要	Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein contained a 22-amino acid signal peptide and a 274-amino acid mature peptide. The Hox6 (AjHBOX6) full-length cDNA contained 1312 bp with a 966-bp open reading frame encoding 321 amino acids. Its deduced amino acid sequence included one homeodomain (60 amino acids) and three domains with low compositional complexity. Comparison and phylogenetic analysis revealed that Wnt6 and Hox6 in A. japonicus had the nearest relationship to those in sea urchins. Further expression analysis by real-time PCR showed that Wnt6 and Hox6 were apparently upregulated during intestine regeneration, which suggested that they were involved in the intestinal regeneration of sea cucumbers.; Many developmental genes play important roles in regeneration. Here, we cloned the two developmental genes, Wnt6 and Hox6, from Apostichopus japonicus by rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The Wnt6 full-length cDNA is 1140 bp long with a 1029-bp open reading frame encoding 342 amino acids. The deduced Wnt6 protein contained a 22-amino acid signal peptide and a 274-amino acid mature peptide. The Hox6 (AjHBOX6) full-length cDNA contained 1312 bp with a 966-bp open reading frame encoding 321 amino acids. Its deduced amino acid sequence included one homeodomain (60 amino acids) and three domains with low compositional complexity. Comparison and phylogenetic analysis revealed that Wnt6 and Hox6 in A. japonicus had the nearest relationship to those in sea urchins. Further expression analysis by real-time PCR showed that Wnt6 and Hox6 were apparently upregulated during intestine regeneration, which suggested that they were involved in the intestinal regeneration of sea cucumbers.
关键词	Wnt6 Hox6 Sea Cucumber Intestinal Regeneration
学科领域	Biochemistry & Molecular Biology ; Genetics & Heredity
DOI	10.4238/2013.November.7.7
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:000331608000121
WOS关键词	GLABERRIMA SELENKA HOLOTHUROIDEA ; VISCERAL REGENERATION ; LIMB MYOGENESIS ; AXOLOTL LIMBS ; GENE ; MECHANISMS ; ROLES ; ECHINODERMATA ; SEQUENCES ; EPENDYMIN
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：23[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16465
专题	海洋生态与环境科学重点实验室
通讯作者	Yang, HS
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, L. N.,Yang, H. S.,Chen, M. Y.,et al. Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus[J]. GENETICS AND MOLECULAR RESEARCH,2013,12(4):5321-5334.
APA	Sun, L. N.,Yang, H. S.,Chen, M. Y.,Xu, D. X.,&Yang, HS.(2013).Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus.GENETICS AND MOLECULAR RESEARCH,12(4),5321-5334.
MLA	Sun, L. N.,et al."Cloning and expression analysis of Wnt6 and Hox6 during intestinal regeneration in the sea cucumber Apostichopus japonicus".GENETICS AND MOLECULAR RESEARCH 12.4(2013):5321-5334.

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