Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei

doi:10.1111/are.14522

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei
	Fang, Han1,2 ; Liu, Xinwei 3; Shao, Xuqing 4; Xin, Fang1,2 ; Wang, Baojie 1,5,6; Wang, Mengqiang1,5,6 ; Jiang, Keyong 1,5,6; Liu, Mei 1,5,6; Wang, Lei 1,5,6
	2020-01-23
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
页码	11
通讯作者	Liu, Mei(liumei@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
摘要	Raptor, a member of the target of rapamycin complex 1 (TORC1), participates in the formation of complex proteins related to the mechanistic target of rapamycin (mTOR) signalling. In this study, a 5,020 bp cDNA of Raptor with an open reading frame (ORF) of 3,804 bp encoding for 1,267 amino acids was cloned from Litopenaeus vannamei. The protein contains three conserved domains: Raptor N, HEAT and WD40 domains. The expression of Raptor gene was detected by qRT-PCR in different tissues of L. vannamei, including hepatopancreas, intestinal, stomach, eyestalk, gill and muscle. The mRNA expression profiles of Raptor in muscle were also analysed under suppression or stimulation of mTOR signalling pathway. The level of Raptor mRNA significantly increased either at 0.5-6 hr after an injection of rapamycin (RAPA) or after 3 days starvation. Leucine or arginine alleviated the up-regulation of Raptor gene expression caused by RAPA or starvation. The Raptor gene was successfully suppressed using RNA interference (RNAi) technology, and the gene expression and the protein phosphorylation level of 4EBP1 and S6K were significantly decreased. The results of the study suggested that the expression of Raptor was sensitive to the immunology status of L. vannamei and participated in nutritional metabolism.
关键词	gene expression Litopenaeus vannamei molecular characterization Raptor RNA interference
DOI	10.1111/are.14522
收录类别	SCI
语种	英语
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; National Natural Science Foundation of China[41406151] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; National Natural Science Foundation of China[41406151]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:000508760300001
出版者	WILEY
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164395
专题	实验海洋生物学重点实验室
通讯作者	Liu, Mei; Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Amsterdam, Amsterdam, Netherlands 4.Shandong Cigna Detect Technol Co Ltd, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Fang, Han,Liu, Xinwei,Shao, Xuqing,et al. Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei[J]. AQUACULTURE RESEARCH,2020:11.
APA	Fang, Han.,Liu, Xinwei.,Shao, Xuqing.,Xin, Fang.,Wang, Baojie.,...&Wang, Lei.(2020).Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei.AQUACULTURE RESEARCH,11.
MLA	Fang, Han,et al."Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei".AQUACULTURE RESEARCH (2020):11.

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