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Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei
Fang, Han1,2; Liu, Xinwei3; Shao, Xuqing4; Xin, Fang1,2; Wang, Baojie1,5,6; Wang, Mengqiang1,5,6; Jiang, Keyong1,5,6; Liu, Mei1,5,6; Wang, Lei1,5,6
2020-01-23
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Pages11
Corresponding AuthorLiu, Mei(liumei@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
AbstractRaptor, a member of the target of rapamycin complex 1 (TORC1), participates in the formation of complex proteins related to the mechanistic target of rapamycin (mTOR) signalling. In this study, a 5,020 bp cDNA of Raptor with an open reading frame (ORF) of 3,804 bp encoding for 1,267 amino acids was cloned from Litopenaeus vannamei. The protein contains three conserved domains: Raptor N, HEAT and WD40 domains. The expression of Raptor gene was detected by qRT-PCR in different tissues of L. vannamei, including hepatopancreas, intestinal, stomach, eyestalk, gill and muscle. The mRNA expression profiles of Raptor in muscle were also analysed under suppression or stimulation of mTOR signalling pathway. The level of Raptor mRNA significantly increased either at 0.5-6 hr after an injection of rapamycin (RAPA) or after 3 days starvation. Leucine or arginine alleviated the up-regulation of Raptor gene expression caused by RAPA or starvation. The Raptor gene was successfully suppressed using RNA interference (RNAi) technology, and the gene expression and the protein phosphorylation level of 4EBP1 and S6K were significantly decreased. The results of the study suggested that the expression of Raptor was sensitive to the immunology status of L. vannamei and participated in nutritional metabolism.
Keywordgene expression Litopenaeus vannamei molecular characterization Raptor RNA interference
DOI10.1111/are.14522
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; National Natural Science Foundation of China[41406151]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000508760300001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164395
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Mei; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Univ Amsterdam, Amsterdam, Netherlands
4.Shandong Cigna Detect Technol Co Ltd, Qingdao, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Fang, Han,Liu, Xinwei,Shao, Xuqing,et al. Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei[J]. AQUACULTURE RESEARCH,2020:11.
APA Fang, Han.,Liu, Xinwei.,Shao, Xuqing.,Xin, Fang.,Wang, Baojie.,...&Wang, Lei.(2020).Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei.AQUACULTURE RESEARCH,11.
MLA Fang, Han,et al."Molecular and functional characterization of Raptor in mTOR pathway from Litopenaeus vannamei".AQUACULTURE RESEARCH (2020):11.
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