IOCAS-IR  > 实验海洋生物学重点实验室
p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis
Zhang, Shujing1,3,4; Yu, Jiajia1,4; Wang, Hongxia1,2; Liu, Baozhong1,2,4; Yue, Xin1,2
2019-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume95Pages:456-463
Corresponding AuthorYue, Xin(yuexin@qdio.ac.cn)
Abstractp38 mitogen-activated protein kinases (MAPKs) are involved in the response to various extracellular stimuli via regulating gene expression. In the present study, a p38 MAPK gene (MpP38) was identified from the clam Meretrix petechialis. The full-length cDNA of MpP38 measures 1,720 bp, consisting of a 134-bp 5'-UTR, a 1,095-bp ORF and a 491-bp 3'-UTR. Both the mRNA and protein expression levels of MpP38 increased after Vibrio challenge, implying that MpP38 is involved in clam immunity. Based on our previous study, a transcription factor activated by p38 MAPK, i.e. microphthalmia-associated transcription factor (MITF), participated in clam immunity by regulating the expression of phenoloxidase (PO). Coupled with other related reports, the mechanism underlying the involvement of MpP38 in clam immunity is most likely that pathogen stimuli induce the phosphorylation of p38 MAPK and thus activate MITF to regulate the expression of the immune-related gene PO. The results obtained in this study support this mechanism. First, we found that the MpP38 phosphorylation level increased in response to Vibrio challenge. Second, as revealed by a yeast two-hybrid assay, there was a direct interaction between MpP38 and MITF. Meanwhile, inhibiting the phosphorylation of MpP38 decreased the phosphorylation level of MpMITF, implying that MpP38 phosphorylation is required for MpMITF activation. Additionally, our results showed that there was a regulatory relationship between MpP38 phosphorylation level and PO expression level. With increased MpP38 phosphorylation level, the PO expression level was also increased after Vibrio challenge; when MpP38 phosphorylation was inhibited, the PO expression level was significantly decreased. This study describes the immune function of p38 MAPK in the clam for the first time and analyses its potential underlying mechanism, which will help to elucidate the immune mechanism in the clam M. petechialis.
Keywordp38 MAPK Vibrio challenge Immune defence MITF
DOI10.1016/j.fsi.2019.10.048
Indexed BySCI
Language英语
Funding ProjectMarine S&T Fund of Shandong Province (China)[2018SDKJ0502-1] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology (China)[YQ2018N007] ; Agriculture Research System of China[CARS-49] ; National Key R&D Program of China[2019YFD0900700] ; National Natural Science Foundation of China[NSFC 31772845]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000503099200044
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164359
Collection实验海洋生物学重点实验室
Corresponding AuthorYue, Xin
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Shandong, Peoples R China
3.Shandong Univ Technol, Sch Life Sci, Antiaging & Regenerat Med Res Inst, Shandong Prov Key Lab Anim Cell & Dev Biol, Zibo 255049, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Shujing,Yu, Jiajia,Wang, Hongxia,et al. p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:456-463.
APA Zhang, Shujing,Yu, Jiajia,Wang, Hongxia,Liu, Baozhong,&Yue, Xin.(2019).p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis.FISH & SHELLFISH IMMUNOLOGY,95,456-463.
MLA Zhang, Shujing,et al."p38 MAPK is involved in the immune response to pathogenic Vibrio in the clam Meretrix petechialis".FISH & SHELLFISH IMMUNOLOGY 95(2019):456-463.
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