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Reductive degradation of chloramphenicol by Geobacter metallireducens
Xu HengDuo1,2; Xiao LeiLei2; Zheng ShiLing2; Zhang YueChao2,3,4; Li JiaJia2,3,4; Liu FangHua2,3
Corresponding AuthorLiu FangHua(
AbstractGeobacter metallireducens is known to be capable of removing nitroaromatic compounds via an oxidation mode. However, little attention has been paid to investigate the reductive removal of chlorinated nitroaromatic compounds by G. metallireducens. In this study, G. metallireducens was used to reduce chloramphenicol (CAP), a typical chlorinated nitroaromatic antibiotic. Cyclic voltammograms and chronoamperometry highlighted a higher peak current for CAP reduction by G. metallireducens compared to the control without bacteria. G. metallireducens efficiently reduced CAP (20 mg/L) with acetate as the sole electron donor, and the removal efficiency reached (97.6 +/- 4.9)% within 6 d. Aromatic amine (AMCl2), AMCl (dechlorinated AMCl2) and AM (dechlorinated AMCl) were identified as reduction products by liquid chromatography-mass spectrometry. However, the removal efficiency declined to (25.0 +/- 3.6)% when the CAP dosage increased to 80 mg/L. Transcriptomic analysis indicated the significant upregulation of genes related to electron transfer, such as pilus assembly protein gene (2.8 folds), NADH-quinone oxidoreductase subunit K2 gene (4.5 folds) and many c-type cytochrome genes such as cytochrome c biogenesis protein ResB (Gmet 2901, 4.6 folds), cytochrome c (Gmet 0335, 4.4 folds) and cytochrome c7 (Gmet 2902, 3.4 folds). Furthermore, a gene related to chlorinated contaminant removal (Gmet 1046, 5.4 folds) was also upregulated, possibly resulting in enhanced CAP reduction. This work deepened our knowledge of the bioremediation ability of G. metallireducens with respect to environmental contaminants and provided a potential strategy to treat antibiotics with electrochemically active bacteria.
Keywordchloramphenicol Geobacter metallireducens dechlorination extracellular electron transfer reduction
Indexed BySCI
Funding ProjectPostdoctoral Science Foundation of China[2018M632735] ; National Natural Science Foundation of China[91751112] ; National Natural Science Foundation of China[41573071] ; Senior User Project of RV KEXUE[KEXUE2018G01] ; Natural Science Foundation[JQ201608] ; Young Taishan Scholars Program of Shandong Province[tsqn20161054]
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Multidisciplinary ; Materials Science, Multidisciplinary
WOS IDWOS:000504097800004
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorLiu FangHua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Xu HengDuo,Xiao LeiLei,Zheng ShiLing,et al. Reductive degradation of chloramphenicol by Geobacter metallireducens[J]. SCIENCE CHINA-TECHNOLOGICAL SCIENCES,2019,62(10):1688-1694.
APA Xu HengDuo,Xiao LeiLei,Zheng ShiLing,Zhang YueChao,Li JiaJia,&Liu FangHua.(2019).Reductive degradation of chloramphenicol by Geobacter metallireducens.SCIENCE CHINA-TECHNOLOGICAL SCIENCES,62(10),1688-1694.
MLA Xu HengDuo,et al."Reductive degradation of chloramphenicol by Geobacter metallireducens".SCIENCE CHINA-TECHNOLOGICAL SCIENCES 62.10(2019):1688-1694.
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