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Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei
Guo, Qing1; Li, Shihao1,2,3; Lv, Xinjia1,4; Xiang, Jianhai1,2; Manor, Rivka5,6; Sagi, Amir5,6; Li, Fuhua1,2,3
2019-12-20
Source PublicationFRONTIERS IN PHYSIOLOGY
ISSN1664-042X
Volume10Pages:14
Corresponding AuthorLi, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cnl)
AbstractThe "eyestalk-androgenic gland (AG)-testis" endocrine axis is involved in male sexual differentiation of crustaceans. The insulin-like androgenic gland hormone (IAG), secreted from the AG, plays a central role in this axis, however key factors upstream the IAG are still poorly understood. Here, two crustacean hyperglycemic hormone (CHH) genes (LvCHH1 and LvCHH2) and their putative receptor guanylate cyclase (LvGC) were identified in Litopenaeus vannamei. LvCHH1 and LvCHH2 belonged to CHH subfamily I members and LvGC was a membrane-bound guanylate cyclase. They were all differentially expressed in eyestalks and gonads of males and females. RNA interference (RNAi) of either LvCHH1 or LvCHH2 increased LvIAG expression, while injection of their recombinant protein decreased LvIAG expression, indicating that LvCHH1 and LvCHH2 are inhibitory factors of LvIAG expression. Yeast two-hybrid assay showed that both LvCHH1 and LvCHH2 interacted with LvGC and their RNAi and recombinant protein injection exerted opposite regulatory effects on the transcriptional expression of LvGC. Meanwhile, knockdown of LvGC increased LvIAG expression. These results suggest that LvGC is the receptor of LvCHH1 and LvCHH2 and they are all involved in male sexual development by regulating LvIAG expression. The present study unveils missing upstream elements in the "eyestalk-AG-testis" endocrine axis in crustacean.
KeywordCHH "eyestalk-AG-testis" endocrine axis guanylate cyclase IAG male sexual differentiation
DOI10.3389/fphys.2019.01525
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0900502] ; National Natural Science Foundation of China[31861143047] ; ISF-NSFC Joint Research Program[2368/18] ; China Agriculture Research System-48[CARS-48]
WOS Research AreaPhysiology
WOS SubjectPhysiology
WOS IDWOS:000505705400001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164203
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Shihao; Li, Fuhua
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel
6.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Guo, Qing,Li, Shihao,Lv, Xinjia,et al. Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei[J]. FRONTIERS IN PHYSIOLOGY,2019,10:14.
APA Guo, Qing.,Li, Shihao.,Lv, Xinjia.,Xiang, Jianhai.,Manor, Rivka.,...&Li, Fuhua.(2019).Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei.FRONTIERS IN PHYSIOLOGY,10,14.
MLA Guo, Qing,et al."Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei".FRONTIERS IN PHYSIOLOGY 10(2019):14.
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