Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei

doi:10.3389/fphys.2019.01525

IOCAS-IR > 实验海洋生物学重点实验室

	Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei
	Guo, Qing 1; Li, Shihao 1,2,3; Lv, Xinjia1,4 ; Xiang, Jianhai1,2 ; Manor, Rivka 5,6; Sagi, Amir 5,6; Li, Fuhua 1,2,3
	2019-12-20
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	10 页码:14
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cnl)
摘要	The "eyestalk-androgenic gland (AG)-testis" endocrine axis is involved in male sexual differentiation of crustaceans. The insulin-like androgenic gland hormone (IAG), secreted from the AG, plays a central role in this axis, however key factors upstream the IAG are still poorly understood. Here, two crustacean hyperglycemic hormone (CHH) genes (LvCHH1 and LvCHH2) and their putative receptor guanylate cyclase (LvGC) were identified in Litopenaeus vannamei. LvCHH1 and LvCHH2 belonged to CHH subfamily I members and LvGC was a membrane-bound guanylate cyclase. They were all differentially expressed in eyestalks and gonads of males and females. RNA interference (RNAi) of either LvCHH1 or LvCHH2 increased LvIAG expression, while injection of their recombinant protein decreased LvIAG expression, indicating that LvCHH1 and LvCHH2 are inhibitory factors of LvIAG expression. Yeast two-hybrid assay showed that both LvCHH1 and LvCHH2 interacted with LvGC and their RNAi and recombinant protein injection exerted opposite regulatory effects on the transcriptional expression of LvGC. Meanwhile, knockdown of LvGC increased LvIAG expression. These results suggest that LvGC is the receptor of LvCHH1 and LvCHH2 and they are all involved in male sexual development by regulating LvIAG expression. The present study unveils missing upstream elements in the "eyestalk-AG-testis" endocrine axis in crustacean.
关键词	CHH "eyestalk-AG-testis" endocrine axis guanylate cyclase IAG male sexual differentiation
DOI	10.3389/fphys.2019.01525
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0900502] ; National Natural Science Foundation of China[31861143047] ; ISF-NSFC Joint Research Program[2368/18] ; China Agriculture Research System-48[CARS-48] ; National Key Research and Development Program of China[2018YFD0900502] ; National Natural Science Foundation of China[31861143047] ; ISF-NSFC Joint Research Program[2368/18] ; China Agriculture Research System-48[CARS-48]
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:000505705400001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164203
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel 6.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Guo, Qing,Li, Shihao,Lv, Xinjia,et al. Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei[J]. FRONTIERS IN PHYSIOLOGY,2019,10:14.
APA	Guo, Qing.,Li, Shihao.,Lv, Xinjia.,Xiang, Jianhai.,Manor, Rivka.,...&Li, Fuhua.(2019).Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei.FRONTIERS IN PHYSIOLOGY,10,14.
MLA	Guo, Qing,et al."Sex-Biased CHHs and Their Putative Receptor Regulate the Expression of IAG Gene in the Shrimp Litopenaeus vannamei".FRONTIERS IN PHYSIOLOGY 10(2019):14.

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