IOCAS-IR  > 海洋生态与环境科学重点实验室
Development of a sensitive qPCR method for the detection of pelagophyte Aureococcus anophagefferens
Zhang, Qing-Chun1,2,3; Chen, Zhen-Fan1,4; Zhao, Jia-Yu1,4; Yu, Ren-Cheng1,2,3,4; Qiu, Li-Mei3,5,6; Kong, Fan-Zhou1,2,3; Wang, Yun-Feng1; Yan, Tian1,2,3; Zhou, Ming-Jiang1
2019-12-20
Source PublicationLIMNOLOGY AND OCEANOGRAPHY-METHODS
ISSN1541-5856
Pages11
AbstractBrown tides of pelagophyte Aureococcus anophagefferens have been recorded in the coastal waters of Qinhuangdao in the Bohai Sea (BS) since 2009. Current understandings on the dynamics and mechanisms of brown tides in the BS, however, are still quite limited due to the lack of accurate methods for detecting low-abundance of A. anophagefferens cells. In this study, a Taqman qPCR method was developed, which targeted 28S rDNA D1-D2 domain of A. anophagefferens. The specificity of the qPCR method was tested using 25 strains of microalgae commonly present in the coastal waters, and the targeted species A. anophagefferens was the only species that had reliable positive signal. The method was highly sensitive and reproducible, and the lower detection limit could reach 0.3 cells of A. anophagefferens. A calibration curve was established for A. anophagefferens using the DNA template extracted from different number of cells, and the lower and upper limits of detection for the qPCR assay is 0.3 cells and 1.2 x 10(5) cells, respectively. A high recovery rate ranged from 96.5% to 107% was achieved for A. anophagefferens at different cell densities. The qPCR assay has been successfully applied to the study on spatial and temporal variations of A. anophagefferens in the coastal waters of Qinhuangdao in 2014.
DOI10.1002/lom3.10344
Indexed BySCI
Language英语
Funding ProjectNational key R&D Program of China[2017YFC1404304] ; National Natural Science Foundation of China[U1606404] ; National Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41276117] ; Program of the Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02] ; National Key Research and Development Program Sino-Australian Centre for Healthy Coasts[2016YFE0101500] ; S&T Basic Resources Investigation Program of China[2018YF100206]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000503554100001
PublisherWILEY
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/164179
Collection海洋生态与环境科学重点实验室
实验海洋生物学重点实验室
Corresponding AuthorYu, Ren-Cheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Marine Biol & Biotechnol Lab, Qingdao, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Qing-Chun,Chen, Zhen-Fan,Zhao, Jia-Yu,et al. Development of a sensitive qPCR method for the detection of pelagophyte Aureococcus anophagefferens[J]. LIMNOLOGY AND OCEANOGRAPHY-METHODS,2019:11.
APA Zhang, Qing-Chun.,Chen, Zhen-Fan.,Zhao, Jia-Yu.,Yu, Ren-Cheng.,Qiu, Li-Mei.,...&Zhou, Ming-Jiang.(2019).Development of a sensitive qPCR method for the detection of pelagophyte Aureococcus anophagefferens.LIMNOLOGY AND OCEANOGRAPHY-METHODS,11.
MLA Zhang, Qing-Chun,et al."Development of a sensitive qPCR method for the detection of pelagophyte Aureococcus anophagefferens".LIMNOLOGY AND OCEANOGRAPHY-METHODS (2019):11.
Files in This Item:
File Name/Size DocType Version Access License
Development of a sen(666KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Qing-Chun]'s Articles
[Chen, Zhen-Fan]'s Articles
[Zhao, Jia-Yu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Qing-Chun]'s Articles
[Chen, Zhen-Fan]'s Articles
[Zhao, Jia-Yu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Qing-Chun]'s Articles
[Chen, Zhen-Fan]'s Articles
[Zhao, Jia-Yu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Development of a sensitive qPCR method for the detection of pelagophyte Aureococcus anophagefferens.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.