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Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine
Yang, Yue1,2,3; Xing, Ronge1,2; Liu, Song1,2; Qin, Yukun1,2; Li, Kecheng1,2; Yu, Huahua1,2; Li, Pengcheng1,2
2020-02-01
Source PublicationCARBOHYDRATE POLYMERS
ISSN0144-8617
Volume229Pages:9
Corresponding AuthorXing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
AbstractChitosan (CS) and its water-soluble derivatives, hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and sulfated chitosan (SCS), were used as adjuvants of inactivated Newcastle disease (ND) vaccine. First, NDV-loaded and blank CS, HACC/CS and SCS nanoparticles were prepared. The particle sizes were respectively 343.43 +/- 4.12, 320.03 +/- 0.84, 156.2 +/- 9.29 nm and the zeta potentials were respectively +19.67 +/- 0.58, +18.3 +/- 0.5, -17.8 +/- 2.65 mV under the optimal conditions. Then chickens were immunized with nanoparticles or commercial inactivated oil emulsion vaccine. After immunization, the humoral immunity levels of the chickens were evaluated. The cellular immunity levels were determined by the quantification of cytokines, lymphocyte proliferation assay, the percentages of CD4(+) and CD8(+) T lymphocytes. Finally, the chickens were challenged with highly virulent virus. The results demonstrated that the humoral immunity levels in NDV-loaded CS and HACC/CS nanoparticles groups were lower than commercial vaccine but the cellular immunity levels are better. Moreover, the prevention effects of NDV-loaded CS and HACC/CS nanoparticles against highly virulent NDV are comparable to commercial vaccine. Our study provides the basis of developing HACC and CS as effective vaccine adjuvants.
KeywordNewcastle disease virus Adjuvant Nanoparticle Chitosan Hydroxypropyltrimethyl ammonium chloride chitosan Sulfated chitosan
DOI10.1016/j.carbpol.2019.115423
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Special projects of Foshan science and technology innovation team[2017IT100054]
WOS Research AreaChemistry ; Polymer Science
WOS SubjectChemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS IDWOS:000501796600026
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164170
Collection实验海洋生物学重点实验室
Corresponding AuthorXing, Ronge; Li, Pengcheng
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Yue,Xing, Ronge,Liu, Song,et al. Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine[J]. CARBOHYDRATE POLYMERS,2020,229:9.
APA Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2020).Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine.CARBOHYDRATE POLYMERS,229,9.
MLA Yang, Yue,et al."Chitosan, hydroxypropyltrimethyl ammonium chloride chitosan and sulfated chitosan nanoparticles as adjuvants for inactivated Newcastle disease vaccine".CARBOHYDRATE POLYMERS 229(2020):9.
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