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Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney
Lou Yanan1,2,3; Sun Bin4; Zhang Lina1,2; Li Yong1,2; Xiao Peng1,2
2019-12-23
发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
页码11
摘要In this study, we established a new cell line (Cynoglossus semilaevis kidney cells, CSK) from kidney of the half-smooth tongue sole (Cynoglossus semilaevis). The cells were subcultured over 1 000 days and passaged for more than 100 times. Additionally, CSK cells were optimally maintained in Dulbecco's modified Eagle medium nutrient mixture F-12 supplemented with HEPES, antibiotics, fetal bovine serum, 2-mercaptoethanol, and basic fibroblast growth factor. The optimum growth temperature for CSK cells was 25 degrees C, and the cells showed a fibroblast-like phenotype. Chromosome analysis revealed that CSK cells had a normal diploid karyotype with 2n=42. CSK cells were susceptible to Grouper nervous necrosis virus (NNV), and cytopathic effects were observed at 3-5 days postinfection. The NNV sensitivity of CSK cells was related to the high abundance of virions in the cytoplasm, as observed by electron microscopy. Additionally, CSK cells could be successfully transfected with a green fluorescent protein reporter plasmid, and fluorescent signals were easily observed. Finally, immunocytochemistry analysis showed that CSK cells were supporting cells. Overall, we established this new cell line, which may have potential applications in the identification of viral pathogens affecting the half-smooth tongue sole.
关键词kidney cell line Cynoglossus semilaevis viral multiplication
DOI10.1007/s00343-019-9084-y
收录类别SCI
语种英语
资助项目Shinan District Project of Science and Technology Development Fund of Qingdao[2016-3-013-ZH] ; National Natural Science Foundation of China[31672692] ; National Natural Science Foundation of China[31672692] ; Shinan District Project of Science and Technology Development Fund of Qingdao[2016-3-013-ZH]
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS类目Limnology ; Oceanography
WOS记录号WOS:000504123300006
出版者SCIENCE PRESS
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/164158
专题实验海洋生物学重点实验室
通讯作者Xiao Peng
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Tianjin Weishan Rd Middle Sch, Tianjin 300222, Peoples R China
第一作者单位中国科学院海洋研究所
通讯作者单位中国科学院海洋研究所
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Lou Yanan,Sun Bin,Zhang Lina,et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019:11.
APA Lou Yanan,Sun Bin,Zhang Lina,Li Yong,&Xiao Peng.(2019).Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,11.
MLA Lou Yanan,et al."Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2019):11.
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