IOCAS-IR  > 实验海洋生物学重点实验室
Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney
Lou Yanan1,2,3; Sun Bin4; Zhang Lina1,2; Li Yong1,2; Xiao Peng1,2
2019-12-23
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Pages11
AbstractIn this study, we established a new cell line (Cynoglossus semilaevis kidney cells, CSK) from kidney of the half-smooth tongue sole (Cynoglossus semilaevis). The cells were subcultured over 1 000 days and passaged for more than 100 times. Additionally, CSK cells were optimally maintained in Dulbecco's modified Eagle medium nutrient mixture F-12 supplemented with HEPES, antibiotics, fetal bovine serum, 2-mercaptoethanol, and basic fibroblast growth factor. The optimum growth temperature for CSK cells was 25 degrees C, and the cells showed a fibroblast-like phenotype. Chromosome analysis revealed that CSK cells had a normal diploid karyotype with 2n=42. CSK cells were susceptible to Grouper nervous necrosis virus (NNV), and cytopathic effects were observed at 3-5 days postinfection. The NNV sensitivity of CSK cells was related to the high abundance of virions in the cytoplasm, as observed by electron microscopy. Additionally, CSK cells could be successfully transfected with a green fluorescent protein reporter plasmid, and fluorescent signals were easily observed. Finally, immunocytochemistry analysis showed that CSK cells were supporting cells. Overall, we established this new cell line, which may have potential applications in the identification of viral pathogens affecting the half-smooth tongue sole.
Keywordkidney cell line Cynoglossus semilaevis viral multiplication
DOI10.1007/s00343-019-9084-y
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31672692] ; Shinan District Project of Science and Technology Development Fund of Qingdao[2016-3-013-ZH]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000504123300006
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/164158
Collection实验海洋生物学重点实验室
Corresponding AuthorXiao Peng
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Tianjin Weishan Rd Middle Sch, Tianjin 300222, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Lou Yanan,Sun Bin,Zhang Lina,et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019:11.
APA Lou Yanan,Sun Bin,Zhang Lina,Li Yong,&Xiao Peng.(2019).Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,11.
MLA Lou Yanan,et al."Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2019):11.
Files in This Item:
File Name/Size DocType Version Access License
Establishment and ch(4173KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lou Yanan]'s Articles
[Sun Bin]'s Articles
[Zhang Lina]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lou Yanan]'s Articles
[Sun Bin]'s Articles
[Zhang Lina]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lou Yanan]'s Articles
[Sun Bin]'s Articles
[Zhang Lina]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidneyharacterizati.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.