Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney | |
Lou Yanan1,2,3; Sun Bin4 ; Zhang Lina1,2; Li Yong1,2; Xiao Peng1,2
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| 2019-12-23 | |
| Source Publication | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 2096-5508 |
| Pages | 11 |
| Corresponding Author | Xiao Peng(xiaopeng@qdio.ac.cn) |
| Abstract | In this study, we established a new cell line (Cynoglossus semilaevis kidney cells, CSK) from kidney of the half-smooth tongue sole (Cynoglossus semilaevis). The cells were subcultured over 1 000 days and passaged for more than 100 times. Additionally, CSK cells were optimally maintained in Dulbecco's modified Eagle medium nutrient mixture F-12 supplemented with HEPES, antibiotics, fetal bovine serum, 2-mercaptoethanol, and basic fibroblast growth factor. The optimum growth temperature for CSK cells was 25 degrees C, and the cells showed a fibroblast-like phenotype. Chromosome analysis revealed that CSK cells had a normal diploid karyotype with 2n=42. CSK cells were susceptible to Grouper nervous necrosis virus (NNV), and cytopathic effects were observed at 3-5 days postinfection. The NNV sensitivity of CSK cells was related to the high abundance of virions in the cytoplasm, as observed by electron microscopy. Additionally, CSK cells could be successfully transfected with a green fluorescent protein reporter plasmid, and fluorescent signals were easily observed. Finally, immunocytochemistry analysis showed that CSK cells were supporting cells. Overall, we established this new cell line, which may have potential applications in the identification of viral pathogens affecting the half-smooth tongue sole. |
| Keyword | kidney cell line Cynoglossus semilaevis viral multiplication |
| DOI | 10.1007/s00343-019-9084-y |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31672692] ; Shinan District Project of Science and Technology Development Fund of Qingdao[2016-3-013-ZH] |
| WOS Research Area | Marine & Freshwater Biology ; Oceanography |
| WOS Subject | Limnology ; Oceanography |
| WOS ID | WOS:000504123300006 |
| Publisher | SCIENCE PRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/164158 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Xiao Peng |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Tianjin Weishan Rd Middle Sch, Tianjin 300222, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Lou Yanan,Sun Bin,Zhang Lina,et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019:11. |
| APA | Lou Yanan,Sun Bin,Zhang Lina,Li Yong,&Xiao Peng.(2019).Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,11. |
| MLA | Lou Yanan,et al."Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2019):11. |
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