Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms | |
Zhang, Yimeng1,2,3,4; Ma, Yan1,3,4; Zhang, Ruiyong5; Zhang, Binbin1,3,4 ; Zhai, Xiaofan1,3,4; Li, Wangqiang1,3,4; Xu, Liting1,3,4; Jiang, Quantong1,3,4; Duan, Jizhou1,3,4; Hou, Baorong1,3,4
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| 2019-12-11 | |
| Source Publication | FRONTIERS IN MICROBIOLOGY
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| ISSN | 1664-302X |
| Volume | 10Pages:13 |
| Corresponding Author | Duan, Jizhou(duanjz@qdio.ac.cn) |
| Abstract | We used metagenomic sequencing combined with morphological and chemical analyses to investigate microbial taxa and functions related to copper-resistance and microbiologically influenced corrosion in mature copper-associated biofilms in coastal seawater for 44 months. Facultative anaerobic microbes such as Woeseia sp. were found to be the dominant groups on the copper surface. Genes related to stress response and possible heavy metal transport systems, especially RNA polymerase sigma factors (rpoE) and putative ATP-binding cassette (ABC) transport system permease protein (ABC.CD.P) were observed to be highly enriched in copper-associated biofilms, while genes encoding DNA-methyltransferase and RNA polymerase subunit were highly enriched in aluminum-associated biofilms and seawater planktonic cells, respectively. Moreover, copper-associated biofilms harbored abundant copper-resistance genes including cus, cop and pco, as well as abundant genes related to extracellular polymeric substances, indicating the presence of diverse copper-resistance patterns. The proportion of dsr in copper-associated biofilms, key genes related to sulfide production, was as low as that in aluminum biofilm and seawater, which ruled out the possibility of microbial sulfide-induced copper-corrosion under field conditions. These results may fill knowledge gaps about the in situ microbial functions of marine biofilms and their effects on toxic-metal corrosion. |
| Keyword | marine biofilm biocorrosion copper-resistance metal alloy gene biofouling |
| DOI | 10.3389/fmicb.2019.02863 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41576080] ; Key Research and Development Plan of Shandong Province[2018GHY115003] ; Key Research and Development Plan of Shandong Province[2018GHY115029] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA13040403] ; Fundamental Research Project of Technology Program of Qingdao[17-1-1-76-JCH] |
| WOS Research Area | Microbiology |
| WOS Subject | Microbiology |
| WOS ID | WOS:000504235400001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/164152 |
| Collection | 海洋环境腐蚀与与生物污损重点实验室 |
| Corresponding Author | Duan, Jizhou |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 5.Fed Inst Geosci & Nat Resources, Hannover, Germany |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhang, Yimeng,Ma, Yan,Zhang, Ruiyong,et al. Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms[J]. FRONTIERS IN MICROBIOLOGY,2019,10:13. |
| APA | Zhang, Yimeng.,Ma, Yan.,Zhang, Ruiyong.,Zhang, Binbin.,Zhai, Xiaofan.,...&Hou, Baorong.(2019).Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms.FRONTIERS IN MICROBIOLOGY,10,13. |
| MLA | Zhang, Yimeng,et al."Metagenomic Resolution of Functional Diversity in Copper Surface-Associated Marine Biofilms".FRONTIERS IN MICROBIOLOGY 10(2019):13. |
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