IOCAS-IR  > 实验海洋生物学重点实验室
Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis
Shao, Zhanru1,2; Zhang, Pengyan1,2,3; Lu, Chang1,2,4; Li, Shaoxuan5; Chen, Zhihang1,2,4; Wang, Xiuliang1,2; Duan, Delin1,2,6
2019-12-12
Source PublicationBMC GENOMICS
ISSN1471-2164
Volume20Issue:1Pages:15
Corresponding AuthorDuan, Delin(dlduan@qdio.ac.cn)
AbstractBackground Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in the brown seaweed Saccharina japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown. Results With WGCNA and trend analysis of 20,940 known genes and 4264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and "imm upregulated 3" gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and Fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. japonica developments.
KeywordAlginate Mannitol Transcriptome Regulatory networks Growth Development Saccharina japonica
DOI10.1186/s12864-019-6366-x
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41806175] ; Shandong Key Research and Development Program[2018GHY115023] ; Qingdao National Laboratory for Marine Science and Technology Project[2018ASKJ03] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1702]
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000502786600002
PublisherBMC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164146
Collection实验海洋生物学重点实验室
Corresponding AuthorDuan, Delin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
5.Qingdao Acad Agr Sci, Qingdao 266100, Shandong, Peoples R China
6.Qingdao Brightmoon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shao, Zhanru,Zhang, Pengyan,Lu, Chang,et al. Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis[J]. BMC GENOMICS,2019,20(1):15.
APA Shao, Zhanru.,Zhang, Pengyan.,Lu, Chang.,Li, Shaoxuan.,Chen, Zhihang.,...&Duan, Delin.(2019).Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis.BMC GENOMICS,20(1),15.
MLA Shao, Zhanru,et al."Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis".BMC GENOMICS 20.1(2019):15.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shao, Zhanru]'s Articles
[Zhang, Pengyan]'s Articles
[Lu, Chang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shao, Zhanru]'s Articles
[Zhang, Pengyan]'s Articles
[Lu, Chang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shao, Zhanru]'s Articles
[Zhang, Pengyan]'s Articles
[Lu, Chang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.