Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis | |
Shao, Zhanru1,2; Zhang, Pengyan1,2,3 ; Lu, Chang1,2,4; Li, Shaoxuan5; Chen, Zhihang1,2,4; Wang, Xiuliang1,2; Duan, Delin1,2,6
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| 2019-12-12 | |
| Source Publication | BMC GENOMICS
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| ISSN | 1471-2164 |
| Volume | 20Issue:1Pages:15 |
| Abstract | Background Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in the brown seaweed Saccharina japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown. Results With WGCNA and trend analysis of 20,940 known genes and 4264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and "imm upregulated 3" gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and Fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. japonica developments. |
| Keyword | Alginate Mannitol Transcriptome Regulatory networks Growth Development Saccharina japonica |
| DOI | 10.1186/s12864-019-6366-x |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1702] ; National Natural Science Foundation of China[41806175] ; Qingdao National Laboratory for Marine Science and Technology Project[2018ASKJ03] ; Shandong Key Research and Development Program[2018GHY115023] ; Shandong Key Research and Development Program[2018GHY115023] ; Qingdao National Laboratory for Marine Science and Technology Project[2018ASKJ03] ; National Natural Science Foundation of China[41806175] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1702] |
| WOS Research Area | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS Subject | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS ID | WOS:000502786600002 |
| Publisher | BMC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/164146 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Duan, Delin |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 5.Qingdao Acad Agr Sci, Qingdao 266100, Shandong, Peoples R China 6.Qingdao Brightmoon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Shandong, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Shao, Zhanru,Zhang, Pengyan,Lu, Chang,et al. Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis[J]. BMC GENOMICS,2019,20(1):15. |
| APA | Shao, Zhanru.,Zhang, Pengyan.,Lu, Chang.,Li, Shaoxuan.,Chen, Zhihang.,...&Duan, Delin.(2019).Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis.BMC GENOMICS,20(1),15. |
| MLA | Shao, Zhanru,et al."Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis".BMC GENOMICS 20.1(2019):15. |
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| Transcriptome sequen（2554KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View | |
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