Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Tongue sole (Cynoglossus semilaevis) interleukin 10 plays a negative role in the immune response against bacterial infection | |
Li, Xue-peng1,2 ; Jiang, Shuai1,2; Sun, Bin1,2,3; Zhang, Jian1,2
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| 2019-12-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 95Pages:93-104 |
| Corresponding Author | Li, Xue-peng(lixuepeng14@mails.ucas.ac.cn) ; Zhang, Jian(zhangjian@qdio.ac.cn) |
| Abstract | Interleukin-10 (IL-10) is a pleiotropic cytokine and plays a crucial role in immunity. In the current study, we examined the expression patterns and biological functions of tongue sole Cynogiossus semilaevis IL-10 (CsIL-10). CsIL-10 is composed of 186 amino acid residues and shares 46.3%-71.7% identities with other teleost IL-10. Csil-10 expression occurred in multiple tissues and was regulated by bacterial infection. Recombinant CsIL-10 (rCsIL-10) in the form of a dimer bound to a wide range of bacterial species but did not affect bacterial growth. rCsIL-10 could interact with peripheral blood leukocytes (PBL) and significantly reduce the phagocytic activity, ROS production, and apoptosis of PBL. When injected in vivo, rCsIL-10 significantly suppressed the expression of proinflammatory cytokines and promoted bacterial dissemination in tongue sole tissues. Consistently, knock-down of Csil-10 significantly inhibited bacterial infection in tongue sole. Taken together, these results indicate that CsIL-10 plays a negative regulatory role in the immune response against bacterial infection. |
| Keyword | Interleukin-10 Cynoglossus semilaevis Peripheral blood leukocytes Immune defense Bacterial infection |
| DOI | 10.1016/j.fsi.2019.10.023 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31802329] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; China Postdoctoral Science Foundation[2018M632733] ; Taishan Scholar Program of Shandong Province ; National Natural Science Foundation of China[31802329] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; China Postdoctoral Science Foundation[2018M632733] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000503099200008 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/164143 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Li, Xue-peng; Zhang, Jian |
| Affiliation | 1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Li, Xue-peng,Jiang, Shuai,Sun, Bin,et al. Tongue sole (Cynoglossus semilaevis) interleukin 10 plays a negative role in the immune response against bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:93-104. |
| APA | Li, Xue-peng,Jiang, Shuai,Sun, Bin,&Zhang, Jian.(2019).Tongue sole (Cynoglossus semilaevis) interleukin 10 plays a negative role in the immune response against bacterial infection.FISH & SHELLFISH IMMUNOLOGY,95,93-104. |
| MLA | Li, Xue-peng,et al."Tongue sole (Cynoglossus semilaevis) interleukin 10 plays a negative role in the immune response against bacterial infection".FISH & SHELLFISH IMMUNOLOGY 95(2019):93-104. |
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| Tongue sole (Cynoglo（1861KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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