IOCAS-IR  > 海洋生态与环境科学重点实验室
Time course analysis of immunity-related gene expression in the sea cucumber Apostichopus japonicus during exposure to thermal and hypoxic stress
Huo, Da1,2,3,4; Sun, Lina1,2,3,4; Zhang, Libin1,2,3,4; Yang, Hongsheng1,2,3,4; Liu, Shilin1,2,4; Sun, Jingchun1,2,4; Su, Fang1,2,3,4
2019-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume95Pages:383-390
Corresponding AuthorSun, Lina(sunlina@qdio.ac.cn) ; Yang, Hongsheng(hshyang@ms.qdio.ac.cn)
AbstractTemperature and dissolved oxygen concentration are important abiotic factors that can limit the growth and survival of sea cucumbers by affecting their immune systems. As global warming intensifies, sea cucumbers are increasingly exposed to adverse environmental conditions, which can cause severe economic losses and limit the sustainable development of sea cucumber aquaculture. It is therefore important to better understand how sea cucumbers respond to environmental stress, especially with regard to its effects on immunity. In the present study, the time series of immunity-related gene expression in sea cucumbers under thermal and hypoxic stresses were analyzed separately. The expression trends of 17 genes related to the nuclear factor kappa B (NF-kappa B) pathway, the protease family, the complement system, heat shock proteins (HSPs) and the transferrin family during exposure to two stresses at eight time points were concluded. These genes have interconnected roles in stress defense. The expression levels of genes relating to the NF-kappa B pathways and HSPs were strongly affected in the sea cucumber thermal stress response, while melanotransferrin (MO), ferritin (Ft) and mannan-binding C-type lectin (MBCL) were affected by hypoxia. In contrast, complement factor B (Bf), myosin V (Mys) and serine protease inhibitor (SPI) were not that sensitive during the initial period of environmental stress. Similar expression patterns under both thermal and hypoxic stress for certain genes, including an increase in Hsp90 and decreases in lysozyme (Lys), major yolk protein (MYP) and cathepsin C (CTLC) were observed in sea cucumbers. Conversely, NF-kappa B and Hsp70 were differentially affected by the two stress treatments. Lysozyme-induced immune defense was inconstant in sea cucumbers coping with stress. A gene ontology (GO) analysis of the selected genes revealed that the most co-involved terms related to immunity and iron ion. Our analysis suggests that sea cucumbers demonstrate complex and varied immune responses to different types of stresses. This dynamic image of the immune responses and stress tolerance of sea cucumbers provides new insights into the adaptive strategies of holothurians in adverse environments.
KeywordAnoxia Heat stress Global climate change Holothurian Echinoderm Immune system High temperature Immune-associated genes Stress response Aquaculture
DOI10.1016/j.fsi.2019.09.073
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0901602] ; National Natural Science Foundation of China[41776161] ; National Natural Science Foundation of China[41776162] ; Agricultural Seed Project of Shandong Province[2016LZGC032] ; Agricultural Seed Project of Shandong Province[2017LZGC010] ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-09] ; Taishan Scholars Program (Distinguished Taishan Scholars) ; Youth Innovation Promotion Association CAS[2019209]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000503099200037
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164113
Collection海洋生态与环境科学重点实验室
Corresponding AuthorSun, Lina; Yang, Hongsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huo, Da,Sun, Lina,Zhang, Libin,et al. Time course analysis of immunity-related gene expression in the sea cucumber Apostichopus japonicus during exposure to thermal and hypoxic stress[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:383-390.
APA Huo, Da.,Sun, Lina.,Zhang, Libin.,Yang, Hongsheng.,Liu, Shilin.,...&Su, Fang.(2019).Time course analysis of immunity-related gene expression in the sea cucumber Apostichopus japonicus during exposure to thermal and hypoxic stress.FISH & SHELLFISH IMMUNOLOGY,95,383-390.
MLA Huo, Da,et al."Time course analysis of immunity-related gene expression in the sea cucumber Apostichopus japonicus during exposure to thermal and hypoxic stress".FISH & SHELLFISH IMMUNOLOGY 95(2019):383-390.
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