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Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis
Du, Yang1,2; Fang, Han2,3; Shao, Xuqing5; Liu, Mei1,2; Jiang, Keyong1,2; Wang, Mengqiang1,2; Wang, Baojie1,2; Wang, Lei1,2,4
2019-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume95Pages:368-382
Corresponding AuthorWang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(wanglei@qdio.ac.cn)
AbstractOur previous work showed that using Lactobacillus pentosus HC-2 as a probiotic could improve the growth performance, immune response, gut bacterial diversity and disease resistance of Litopenaeus vannamei. However, the probiotic mechanism had not been fully characterized. In the present study, histology and proteomic analysis were performed to explore the influence of HC-2 surface protein on its probiotic effects on L. vannamei after feeding either the intact surface proteins, the probiotic treated with lithium chloride (LiCl) to remove noncovalently bound surface proteins or no probiotic for four weeks. Histological observation found that feeding with normal HC-2 obviously improved the intestinal histology and enhanced the protective effect against pathogen damage, but feeding with LiCl-treated HC-2 did not improve the intestinal environment. A total of over 2764 peptides and 1118 uniproteins were identified from the L. vannamei midgut; 211 proteins were significantly differentially expressed in the normal HC-2 group compared with the control group; 510 proteins were significantly differentially expressed in the LiCl-treated HC-2 group compared with the control group, and 458 proteins were significantly differentially expressed in the LiCl-treated HC-2 group compared with the normal HC-2 group. GO/KEGG enrichment analysis of the significantly different proteins demonstrated that feeding normal HC-2 mainly induced immune response, metabolic, cell adhesion and cell-cell signaling-related protein upregulation, which contributed to bacterial adhesion and colonization in the midgut to improve the shrimp immune system and growth, but these proteins were suppressed after the shrimp were fed bacteria deprived of surface proteins. Taken together, these results indicate that the surface proteins were indispensable for HC-2 to execute probiotic effects in the shrimp midgut.
KeywordLitopenaeus vannamei Lactobacillus pentosus HC-2 Surface proteins Proteomic analysis
DOI10.1016/j.fsi.2019.10.059
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41906107] ; China Postdoctoral Science Foundation[2018M632736] ; Scientific and technological development fund project of Shinan district of Qingdao city[2018-4-001-ZH]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000503099200036
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164110
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Baojie; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China
5.Shandong Cigna Detect Technol Co Ltd, Qingdao 266237, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Yang,Fang, Han,Shao, Xuqing,et al. Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,95:368-382.
APA Du, Yang.,Fang, Han.,Shao, Xuqing.,Liu, Mei.,Jiang, Keyong.,...&Wang, Lei.(2019).Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis.FISH & SHELLFISH IMMUNOLOGY,95,368-382.
MLA Du, Yang,et al."Exploration of the influence of surface proteins on the probiotic activity of Lactobacillus pentosus HC-2 in the Litopenaeus vannamei midgut via label-free quantitative proteomic analysis".FISH & SHELLFISH IMMUNOLOGY 95(2019):368-382.
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