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Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei
Liu, Fei1,2; Li, Shihao1,2,3; Yu, Yang1,2,3; Sun, Mingzhe1,2,4; Xiang, Jianhai1,2; Li, Fuhua1,2,3
2020
Source PublicationCHEMOSPHERE
ISSN0045-6535
Volume239Pages:12
Corresponding AuthorLi, Fuhua(fhli@qdio.ac.cn)
AbstractAmmonia is an important environmental stress factor in aquaculture. Long-term ammonia stress could affect the normal growth, and also increase the risk for the occurrence of various diseases. In order to learn the mechanism that ammonia caused the outbreak of the shrimp disease, transcriptomics and metabolomics approaches were used to analyze the differential expressions of the genes in hemocytes and different metabolites in the serum of the Pacific white shrimp Litopenaeus vannamei under ammonia exposure. Transcriptional analysis showed that 17 cell apoptosis related genes, seven phagocytosis related genes, 10 immunity related genes and seven cell cycle and lipid metabolism related genes showed differential expressions after ammonia exposure. Metabolomics analysis on the serum showed that 25 differential metabolites were identified in positive and negative ion patterns. They are involved in purine metabolism, amino acids metabolism and lipid metabolism. Injection of two up-regulated metabolites triethanolamine and oxypurinol to normal shrimp could induce apoptosis in normal shrimp. The total hemocytes counts in shrimp showed a significant decrease and the apoptotic cell ratio increased significantly under ammonia exposure. These results suggested that ammonia exposure increased the apoptosis of hemocytes, which affected the immunity of shrimp, and thus caused susceptibility to pathogenic infection. These data will help us understand the mechanism of ammonia stress leading to the immunity decline of shrimp. (C) 2019 Elsevier Ltd. All rights reserved.
KeywordAmmonia stress Metabolome Transcriptome Hemocytes Litopenaeus vannamei
DOI10.1016/j.chemosphere.2019.124759
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[41776158] ; National Natural Science Foundation of China[31772880] ; China Agriculture Research system-48[CARS-48]
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000498305500068
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163865
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Fei,Li, Shihao,Yu, Yang,et al. Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei[J]. CHEMOSPHERE,2020,239:12.
APA Liu, Fei,Li, Shihao,Yu, Yang,Sun, Mingzhe,Xiang, Jianhai,&Li, Fuhua.(2020).Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei.CHEMOSPHERE,239,12.
MLA Liu, Fei,et al."Effects of ammonia stress on the hemocytes of the Pacific white shrimp Litopenaeus vannamei".CHEMOSPHERE 239(2020):12.
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