Particle and bacteria uptake by Japanese flounder (Paralichthys olivaceus) red blood cells: Size dependence and pathway specificity

doi:10.1016/j.tice.2019.09.002

IOCAS-IR > 实验海洋生物学重点实验室

	Particle and bacteria uptake by Japanese flounder (Paralichthys olivaceus) red blood cells: Size dependence and pathway specificity
	Sun, Bin1,2,3 ; Sun, Yuan-yuan1,2 ; Lia, Xue-peng 1,2; Jiang, Shuai 1,2; Sun, Li 1,2
	2019-12-01
发表期刊	TISSUE & CELL
ISSN	0040-8166
卷号	61 页码:79-88
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Red blood cells (RBCs) are traditionally considered non-professional phagocytes functioning predominately in oxygen transport. In the present study, we examined the ability of Japanese flounder (Paralichthys olivaceus), a teleost species with important economic values, RBCs to uptake inorganic particles and bacteria in different size/form, as well as the involving endocytic pathways. We found that flounder RBCs exhibited relatively high uptake/attachment capacities for 0.1 mu m-1.0 mu m (diameter) latex beads, but not for 2.0 mu m beads. For the 0.1 beads, the uptake/attachment was executed through macropinocytosis and caveolae-mediated pathway, while for 0.5 mu m and 1.0 mu m beads, the uptake/attachment depended primarily on macropinocytosis and partially on the caveolin-mediated pathway. In addition to latex beads, flounder RBCs also exhibited an apparent capacity to engulf both live and inactivated bacteria. For live bacteria, the endocytosis was clathrin-mediated, while for inactivated bacteria, clathrin- as well as non-clathrin-mediated endocytosis were involved. Taken together, these results demonstrated that teleost RBCs possess particle uptake/attachment and bacteria phagocytosis capacities via different pathways that depend on the physical size and biological nature of the engulfed objects.
关键词	Red blood cells/erythrocytes Paralichthys olivaceus Endocytosis Phagocytosis
DOI	10.1016/j.tice.2019.09.002
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900500] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Aoshan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province ; National Key R&D Program of China[2018YFD0900500] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Anatomy & Morphology ; Cell Biology
WOS类目	Anatomy & Morphology ; Cell Biology
WOS记录号	WOS:000497983800011
出版者	CHURCHILL LIVINGSTONE
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/163826
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Bin,Sun, Yuan-yuan,Lia, Xue-peng,et al. Particle and bacteria uptake by Japanese flounder (Paralichthys olivaceus) red blood cells: Size dependence and pathway specificity[J]. TISSUE & CELL,2019,61:79-88.
APA	Sun, Bin,Sun, Yuan-yuan,Lia, Xue-peng,Jiang, Shuai,&Sun, Li.(2019).Particle and bacteria uptake by Japanese flounder (Paralichthys olivaceus) red blood cells: Size dependence and pathway specificity.TISSUE & CELL,61,79-88.
MLA	Sun, Bin,et al."Particle and bacteria uptake by Japanese flounder (Paralichthys olivaceus) red blood cells: Size dependence and pathway specificity".TISSUE & CELL 61(2019):79-88.

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