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Analyses of mRNA-seq and miRNA-seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17 beta-estradiol or 17 alpha-methyltestosterone-induced olive flounder (Paralichthys olivaceus)
Zou, Yuxia1,2; Wu, Zhihao1,2; Fan, Zhaofei1,3; Liang, Dongdong1,3; Wang, Lijuan1,2; Song, Zongcheng4; You, Feng1,2
2019-12-02
Source PublicationMOLECULAR REPRODUCTION AND DEVELOPMENT
ISSN1040-452X
Pages13
Corresponding AuthorYou, Feng(youfeng@qdio.ac.cn)
AbstractOlive flounder (Paralichthys olivaceus) is a commercially important flatfish species cultured in East Asia. Female flounders generally grow more rapidly than males, therefore control of the sex ratio seems to be a proposed way to increase production. However, the sex determination gene and sex determination mechanism have yet been elucidated. The brain is an important organ that is involved in gonadal development. To explore the sex differences of gene expression in the brain before and during the flounder gonadal differentiation, we used messenger RNA (mRNA)-seq technology to investigate transcriptomes of male and female brains. Between female and male brains, 103 genes were differentially expressed before ovarian differentiation, 16 genes were differentially expressed before testicular differentiation, and 64 genes were differentially expressed during gonadal differentiation. According to annotation and Kyoto Encyclopedia of Genes and Genomes information, the differentially expressed genes (DEGs) were involved in circadian rhythm, circadian rhythm-fly, circadian entrainment, dopaminergic synapse, calcium signaling, glutamatergic synapse, taste transduction, herpes simplex infection, long-term depression, retrograde endocannabinoid signaling, and the synaptic vesicle cycle pathways. MicroRNA (miRNA)-seq was performed during the gonadal differentiation and the target genes of miRNAs were predicted. Integrated analysis of mRNA-seq and miRNA-seq showed that 29 of the 64 DEGs were regulated by the differentially expressed miRNAs during the gonadal differentiation. Our study provides a basis for further studies of brain sex differentiation and the molecular mechanism of sex determination in olive flounder.
Keywordbrain gonadal differentiation miRNA-seq mRNA-seq olive flounder (Paralichthys olivaceus)
DOI10.1002/mrd.23303
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Key R & D Program of China[2018YFD0900202] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology ; Developmental Biology ; Reproductive Biology
WOS IDWOS:000499706900001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163817
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
4.Shenghang Aquat Sci & Technol Co Ltd, Weihai, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zou, Yuxia,Wu, Zhihao,Fan, Zhaofei,et al. Analyses of mRNA-seq and miRNA-seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17 beta-estradiol or 17 alpha-methyltestosterone-induced olive flounder (Paralichthys olivaceus)[J]. MOLECULAR REPRODUCTION AND DEVELOPMENT,2019:13.
APA Zou, Yuxia.,Wu, Zhihao.,Fan, Zhaofei.,Liang, Dongdong.,Wang, Lijuan.,...&You, Feng.(2019).Analyses of mRNA-seq and miRNA-seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17 beta-estradiol or 17 alpha-methyltestosterone-induced olive flounder (Paralichthys olivaceus).MOLECULAR REPRODUCTION AND DEVELOPMENT,13.
MLA Zou, Yuxia,et al."Analyses of mRNA-seq and miRNA-seq of the brain reveal the sex differences of gene expression and regulation before and during gonadal differentiation in 17 beta-estradiol or 17 alpha-methyltestosterone-induced olive flounder (Paralichthys olivaceus)".MOLECULAR REPRODUCTION AND DEVELOPMENT (2019):13.
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