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Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai
Gao Xiaolong1,2; Ke Caihuan1; Zhang Mo2; Li Xian2; Wu Fucun2; Liu Ying3
2019-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume94Pages:617-627
Corresponding AuthorLiu Ying(yingliu@dlou.edu.cn)
AbstractThe effects of a diet containing the probiotic Bacillus amyloliquefaciens on the survival and growth of Haliotis discus hannai were evaluated by measuring growth and hematological parameters and the expression levels of nonspecific immune genes. In addition, the abalones response to Vibrio parahaemolyticus infection was assessed. H. discus hannai (shell length: 29.35 +/- 1.81 mm, body weight: 4.28 +/- 0.23 g) were exposed to an 8-week culture experiment in indoor aquariums and a 2-week V. parahaemolyticus artificial infection experiment. In each experiment, the control group (C) was fed daily with the basal feed; the experimental groups were fed daily with the experimental feed, prepared by spraying B. amyloliquefaciens onto the basal feed at final concentrations of 10(3) (group A1), 10(5) (A2), and 10(7) (A3) cfu/g. The survival rate, body weight specific growth rate, and food conversion efficiency in A2 and A3 were significantly higher than those in A1 and C (P < 0.05). The total number of blood lymphocytes, the O2- and NO levels produced from respiratory burst, the activities of acid phosphatase, superoxide dismutase, and catalase, and the expression levels of catalase and thiol peroxidase in A2 were not significantly different from those in A3, but these factors were significantly higher in A2 compared to Al and C (P < 0.05). The total antioxidant capacity and expression levels of glutathione S-transferase in Al, A2 and A3 were significantly higher than those in C (P < 0.05). At day 9 after infection with V. parahaemolyticus, all abalone in C were dead; at the end of the experiment, the cumulative mortality of abalone in A2 was significantly lower than that in any other group (P < 0.05). Thus, the experimental feed containing 10(3) cfu/g B. amyloliquefaciens not only facilitated the food intake and growth of abalone, but also effectively enhanced their non-specific immunity and resistance to V. parahaemolyticus infection. In this regard, B. amyloliquefaciens may be a useful probiotic strain for abalone aquaculture.
KeywordBacillus amyloliquefaciens Haliotis discus hannai Growth Immunity Disease resistance
DOI10.1016/j.fsi.2019.08.067
Indexed BySCI
Language英语
Funding Projectearmarked fund for the Modern Agro-industry Technology Research System[CARS-49] ; China Postdoctoral Science Foundation[2019M650153] ; National Natural Science Foundation of China of China[31672673] ; State Key Laboratory of Marine Environmental Science at Xiamen University
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000496892400065
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163796
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu Ying
Affiliation1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Fujian, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
3.Dalian Ocean Univ, Dalian 116023, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gao Xiaolong,Ke Caihuan,Zhang Mo,et al. Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:617-627.
APA Gao Xiaolong,Ke Caihuan,Zhang Mo,Li Xian,Wu Fucun,&Liu Ying.(2019).Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai.FISH & SHELLFISH IMMUNOLOGY,94,617-627.
MLA Gao Xiaolong,et al."Effects of the probiotic Bacillus amyloliquefaciens on the growth, immunity, and disease resistance of Haliotis discus hannai".FISH & SHELLFISH IMMUNOLOGY 94(2019):617-627.
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