Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine | |
Chen, Chao1,2,3,4 ; Wang, Yi1,3,4; Zhang, Dun1,3,4
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| 2019-12-01 | |
| 发表期刊 | MICROCHIMICA ACTA
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| ISSN | 0026-3672 |
| 卷号 | 186期号:12页码:8 |
| 通讯作者 | Wang, Yi(wangyi@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
| 摘要 | It is demonstrated that vanadium tetrasulfide (VS4) exhibits peroxidase (POx)-like activity which follows Michaelis-Menten kinetics when using H2O2 as a co-substrate. Electron spin resonance spectroscopy was use to analyze the catalytic mechanism. It suggests that the enzyme mimicking activity is caused by decomposing H2O2 into hydroxyl radicals. The method was used to quantify H2O2 by using 3,3 ',5,5 '-tetramethylbenzidine as the substrate which results in the formation of a blue coloration (with an absorption peak at 652 nm). H2O2 can be detected in the 50 to 300 mu M concentration range, and the detection limit is 5.0 mu M. The assay for L-cysteine (L-cys) is based on the capability of oxTMB to oxidize L-cys to form L-cystine. The colorimetric L-cys assay has a linear response in the 5 to 100 mu M concentration range and a 2.5 mu M detection limit. Graphical abstract Schematic representation of the enzyme mimicking activity of vanadium tetrasulfide (VS4) submicrospheres originated from the decomposition of hydrogen peroxide (H2O2) to generate reactive hydroxyl radical (center dot OH) and the colorimetric detection of L-cysteine. |
| 关键词 | VS4 Catalytic TMB Mechanism Nanozyme Hydroxyl radical Colorimetric Detection H2O2 Transition metal chalcogenides |
| DOI | 10.1007/s00604-019-3942-3 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050104] ; Natured Science Foundation of China[41776090] ; Key Research and Development Program of Shandong Province[2018GHY115038] ; Key Research and Development Program of Shandong Province[2018GGX104021] ; AoShan Talent Program ; Qingdao National Laboratory for Marine Science and Technology ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050104] ; Natured Science Foundation of China[41776090] ; Key Research and Development Program of Shandong Province[2018GHY115038] ; Key Research and Development Program of Shandong Province[2018GGX104021] ; AoShan Talent Program ; Qingdao National Laboratory for Marine Science and Technology |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000498188200010 |
| 出版者 | SPRINGER WIEN |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/163793 |
| 专题 | 海洋环境腐蚀与生物污损重点实验室 |
| 通讯作者 | Wang, Yi; Zhang, Dun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Chen, Chao,Wang, Yi,Zhang, Dun. Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine[J]. MICROCHIMICA ACTA,2019,186(12):8. |
| APA | Chen, Chao,Wang, Yi,&Zhang, Dun.(2019).Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine.MICROCHIMICA ACTA,186(12),8. |
| MLA | Chen, Chao,et al."Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine".MICROCHIMICA ACTA 186.12(2019):8. |
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