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Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine
Chen, Chao1,2,3,4; Wang, Yi1,3,4; Zhang, Dun1,3,4
2019-12-01
Source PublicationMICROCHIMICA ACTA
ISSN0026-3672
Volume186Issue:12Pages:8
Corresponding AuthorWang, Yi(wangyi@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
AbstractIt is demonstrated that vanadium tetrasulfide (VS4) exhibits peroxidase (POx)-like activity which follows Michaelis-Menten kinetics when using H2O2 as a co-substrate. Electron spin resonance spectroscopy was use to analyze the catalytic mechanism. It suggests that the enzyme mimicking activity is caused by decomposing H2O2 into hydroxyl radicals. The method was used to quantify H2O2 by using 3,3 ',5,5 '-tetramethylbenzidine as the substrate which results in the formation of a blue coloration (with an absorption peak at 652 nm). H2O2 can be detected in the 50 to 300 mu M concentration range, and the detection limit is 5.0 mu M. The assay for L-cysteine (L-cys) is based on the capability of oxTMB to oxidize L-cys to form L-cystine. The colorimetric L-cys assay has a linear response in the 5 to 100 mu M concentration range and a 2.5 mu M detection limit. Graphical abstract Schematic representation of the enzyme mimicking activity of vanadium tetrasulfide (VS4) submicrospheres originated from the decomposition of hydrogen peroxide (H2O2) to generate reactive hydroxyl radical (center dot OH) and the colorimetric detection of L-cysteine.
KeywordVS4 Catalytic TMB Mechanism Nanozyme Hydroxyl radical Colorimetric Detection H2O2 Transition metal chalcogenides
DOI10.1007/s00604-019-3942-3
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of Chinese Academy of Sciences[XDA23050104] ; Natured Science Foundation of China[41776090] ; Key Research and Development Program of Shandong Province[2018GHY115038] ; Key Research and Development Program of Shandong Province[2018GGX104021] ; AoShan Talent Program ; Qingdao National Laboratory for Marine Science and Technology
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000498188200010
PublisherSPRINGER WIEN
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163793
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorWang, Yi; Zhang, Dun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Chao,Wang, Yi,Zhang, Dun. Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine[J]. MICROCHIMICA ACTA,2019,186(12):8.
APA Chen, Chao,Wang, Yi,&Zhang, Dun.(2019).Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine.MICROCHIMICA ACTA,186(12),8.
MLA Chen, Chao,et al."Peroxidase-like activity of vanadium tetrasulfide submicrospheres and its application to the colorimetric detection of hydrogen peroxide and L-cysteine".MICROCHIMICA ACTA 186.12(2019):8.
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