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Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions
Gong, Fengying1,2; Zhang, Chunhui1,2,3; Zhang, Litao1,3,4; Liu, Jianguo1,3,4
2019-12-04
Source PublicationAQUACULTURE RESEARCH
ISSN1355-557X
Pages9
Corresponding AuthorLiu, Jianguo(jgliu@qdio.ac.cn)
AbstractIn this study, carotenoid contents of Haematococcus pluvialis during outdoor high light cultivation were measured, moreover, changes of astaxanthin geometrical isomers and biotic factors which may affect isomerization were investigated. During the incubation, contents of both astaxanthin and its precursors (zeaxanthin and canthaxanthin) increased over time, whereas the relative content of lutein decreased. Contents of all astaxanthin isomers increased, while the proportion of different astaxanthin geometrical isomers fluctuated during the incubation. All-trans-astaxanthin of total astaxanthin (T/A) was higher during the astaxanthin accumulation phase than that in the cell transformation phase, which was in contrast to results of 13-cis-astaxanthin of total astaxanthin (13C/A) and 9-cis-astaxanthin of total astaxanthin (9C/A). Farnesyl diphosphate synthase (trans (2E,6E)-FPPS, EC2.5.1.10) and geranylgeranyl diphosphate synthase (GGPS, EC2.5.1.29), the key proteins involved in geometrical isomerization, decreased during the astaxanthin accumulation phase. Moreover, the presence of cis (2Z,6E)-FPPS was firstly confirmed in H. pluvialis by HPLC-MS/MS shotgun method. The results indicated the biotic factor (trans-FPPS, cis-FPPS and GGPS) may play an important role in astaxanthin geometrical isomerization of H. pluvialis, but not a crucial role. This study would help in optimizing the regulation of astaxanthin geometrical isomers in H. pluvialis, with great significance in theory and production.
Keywordastaxanthin carotenoids geometrical isomerization haematococcus pluvialis prenyltransferases
DOI10.1111/are.14427
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31572639] ; National Natural Science Foundation of China[U1706209]
WOS Research AreaFisheries
WOS SubjectFisheries
WOS IDWOS:000500304300001
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163790
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Jianguo
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
4.Yunnan Alphy Biotech Co Ltd, Natl Local Joint Engn Res Ctr Haematococcus Pluvi, Chuxiong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gong, Fengying,Zhang, Chunhui,Zhang, Litao,et al. Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions[J]. AQUACULTURE RESEARCH,2019:9.
APA Gong, Fengying,Zhang, Chunhui,Zhang, Litao,&Liu, Jianguo.(2019).Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions.AQUACULTURE RESEARCH,9.
MLA Gong, Fengying,et al."Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions".AQUACULTURE RESEARCH (2019):9.
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