Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions | |
Gong, Fengying1,2 ; Zhang, Chunhui1,2,3; Zhang, Litao1,3,4; Liu, Jianguo1,3,4
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| 2019-12-04 | |
| 发表期刊 | AQUACULTURE RESEARCH
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| ISSN | 1355-557X |
| 页码 | 9 |
| 摘要 | In this study, carotenoid contents of Haematococcus pluvialis during outdoor high light cultivation were measured, moreover, changes of astaxanthin geometrical isomers and biotic factors which may affect isomerization were investigated. During the incubation, contents of both astaxanthin and its precursors (zeaxanthin and canthaxanthin) increased over time, whereas the relative content of lutein decreased. Contents of all astaxanthin isomers increased, while the proportion of different astaxanthin geometrical isomers fluctuated during the incubation. All-trans-astaxanthin of total astaxanthin (T/A) was higher during the astaxanthin accumulation phase than that in the cell transformation phase, which was in contrast to results of 13-cis-astaxanthin of total astaxanthin (13C/A) and 9-cis-astaxanthin of total astaxanthin (9C/A). Farnesyl diphosphate synthase (trans (2E,6E)-FPPS, EC2.5.1.10) and geranylgeranyl diphosphate synthase (GGPS, EC2.5.1.29), the key proteins involved in geometrical isomerization, decreased during the astaxanthin accumulation phase. Moreover, the presence of cis (2Z,6E)-FPPS was firstly confirmed in H. pluvialis by HPLC-MS/MS shotgun method. The results indicated the biotic factor (trans-FPPS, cis-FPPS and GGPS) may play an important role in astaxanthin geometrical isomerization of H. pluvialis, but not a crucial role. This study would help in optimizing the regulation of astaxanthin geometrical isomers in H. pluvialis, with great significance in theory and production. |
| 关键词 | astaxanthin carotenoids geometrical isomerization haematococcus pluvialis prenyltransferases |
| DOI | 10.1111/are.14427 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31572639] ; National Natural Science Foundation of China[U1706209] ; National Natural Science Foundation of China[U1706209] ; National Natural Science Foundation of China[31572639] |
| WOS研究方向 | Fisheries |
| WOS类目 | Fisheries |
| WOS记录号 | WOS:000500304300001 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/163790 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Jianguo |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 4.Yunnan Alphy Biotech Co Ltd, Natl Local Joint Engn Res Ctr Haematococcus Pluvi, Chuxiong, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Gong, Fengying,Zhang, Chunhui,Zhang, Litao,et al. Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions[J]. AQUACULTURE RESEARCH,2019:9. |
| APA | Gong, Fengying,Zhang, Chunhui,Zhang, Litao,&Liu, Jianguo.(2019).Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions.AQUACULTURE RESEARCH,9. |
| MLA | Gong, Fengying,et al."Changes of carotenoids contents and analysis of astaxanthin geometrical isomerization in Haematococcus pluvialis under outdoor high light conditions".AQUACULTURE RESEARCH (2019):9. |
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