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PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles
Liu, Shun1,2,3; Hu, Zi-Min1,2; Zhang, Quansheng4; Yang, Xiaoqi1,2,3; Critchley, Alan T.5; Duan, Delin1,2
2019-11-27
Source PublicationBMC PLANT BIOLOGY
ISSN1471-2229
Volume19Issue:1Pages:14
Corresponding AuthorHu, Zi-Min(huzm@gdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
AbstractBackground Intermittent dehydration caused by tidal changes is one of the most important abiotic factors that intertidal seaweeds must cope with in order to retain normal growth and reproduction. However, the underlying molecular mechanisms for the adaptation of red seaweeds to repeated dehydration-rehydration cycles remain poorly understood. Results We chose the red seaweed Gloiopeltis furcata as a model and simulated natural tidal changes with two consecutive dehydration-rehydration cycles occurring over 24 h in order to gain insight into key molecular pathways and regulation of genes which are associated with dehydration tolerance. Transcription sequencing assembled 32,681 uni-genes (GC content = 55.32%), of which 12,813 were annotated. Weighted gene co-expression network analysis (WGCNA) divided all transcripts into 20 modules, with Coral2 identified as the key module anchoring dehydration-induced genes. Pathways enriched analysis indicated that the ubiquitin-mediated proteolysis pathway (UPP) and phosphatidylinositol (PI) signaling system were crucial for a successful response in G. furcata. Network-establishing and quantitative reverse transcription PCR (qRT-PCR) suggested that genes encoding ubiquitin-protein ligase E3 (E3-1), SUMO-activating enzyme sub-unit 2 (SAE2), calmodulin (CaM) and inositol-1,3,4-trisphosphate 5/6-kinase (ITPK) were the hub genes which responded positively to two successive dehydration treatments. Network-based interactions with hub genes indicated that transcription factor (e.g. TFIID), RNA modification (e.g. DEAH) and osmotic adjustment (e.g. MIP, ABC1, Bam1) were related to these two pathways. Conclusions RNA sequencing-based evidence from G. furcata enriched the informational database for intertidal red seaweeds which face periodic dehydration stress during the low tide period. This provided insights into an increased understanding of how ubiquitin-mediated proteolysis and the phosphatidylinositol signaling system help seaweeds responding to dehydration-rehydration cycles.
KeywordSeaweed Dehydration Ubiquitination Phosphatidylinositol signaling system Weighted gene co-expression network analysis
DOI10.1186/s12870-019-2125-z
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41761144057] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000501294600001
PublisherBMC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163784
Collection实验海洋生物学重点实验室
Corresponding AuthorHu, Zi-Min; Duan, Delin
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China
5.Univ Cape Breton, Verschuren Ctr Sustainabil Energy & Environm, Sydney, NS, Canada
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Shun,Hu, Zi-Min,Zhang, Quansheng,et al. PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles[J]. BMC PLANT BIOLOGY,2019,19(1):14.
APA Liu, Shun,Hu, Zi-Min,Zhang, Quansheng,Yang, Xiaoqi,Critchley, Alan T.,&Duan, Delin.(2019).PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles.BMC PLANT BIOLOGY,19(1),14.
MLA Liu, Shun,et al."PI signal transduction and ubiquitination respond to dehydration stress in the red seaweed Gloiopeltis furcata under successive tidal cycles".BMC PLANT BIOLOGY 19.1(2019):14.
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