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Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53
Guan, Xiao-lu1,2; Zhang, Bao-cun3,4; Sun, Li1,2
2020-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume103Pages:8
Corresponding AuthorSun, Li(Isun@qdio.ac.cn)
AbstractMicroRNAs (miRNAs) are post-transcriptional regulators that play vital roles in diverse physiological processes including immunity. In this study, we investigated the regulatory mechanism and function of a novel Japanese flounder (Paralichthys olivaceus) miRNA, pol-miR-3p-2. pol-miR-3p-2 was responsive in expression to the infection of the bacterial pathogen Edwardsiella tarda. pol-miR-3p-2 negatively regulated the expression of p53 through interaction with the 3'UTR of p53. Overexpression of pol-miR-3p-2 promoted autophagy, resulting in augmented production of LC3-II, while knockdown of p53 increased the level of beclin, a key factor of autophagy. In vivo and in vitro studies showed that E. tarda infection induced autophagy in flounder, and pol-miR-3p-2 inhibited the infectivity of E. tarda. Together these results indicate that pol-miR-3p-2 regulates autophagy through the target gene p53, thus revealing a regulatory link between p53 and autophagy in teleost, and that pol-miR-3p-2 plays an important role in the immune defense against E. tarda.
KeywordmiRNA Paralichthys olivaceus Autophagy Bacterial infection Immune defense
DOI10.1016/j.dci.2019.103531
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31730100] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000500050700027
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163748
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Aarhus Univ, Dept Biomed, Aarhus, Denmark
4.Aarhus Univ, Aarhus Res Ctr Innate Immun, Aarhus, Denmark
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Guan, Xiao-lu,Zhang, Bao-cun,Sun, Li. Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,103:8.
APA Guan, Xiao-lu,Zhang, Bao-cun,&Sun, Li.(2020).Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,103,8.
MLA Guan, Xiao-lu,et al."Japanese flounder pol-miR-3p-2 suppresses Edwardsiella tarda infection by regulation of autophagy via p53".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 103(2020):8.
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