Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays
Qi, Peng1,2; Zhang, Dun1; Wan, Yi1,2; Zhang, D
2013-05-01
发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
卷号181页码:274-279
文章类型Article
摘要This work presents a novel specific detection method for sulfate-reducing bacteria (SRB) based on sulfuration conversion from ZnO arrays to ZnS arrays. The sulfuration process was conducted in two steps. First, ZnO@ZnS intermediate nanocomposite arrays were formed by the sulfuration reaction between ZnO and sulfide, which is the characteristic enzymatic metabolism product of SRB. Next, unconverted ZnO cores were washed away in ammonia buffer solutions, leaving ZnS arrays on the surface of ITO substrates. The array conversion process can be used for SRB detection because the final state of ZnS arrays was highly related with the amount of accumulated sulfide, which was decided by the initial concentration of SRB. Electrochemical impedance spectroscopy has been used to optimize experimental conditions and detect SRB in aqueous solutions. This SRB detection method based on arrays conversion from ZnO to ZnS can avoid the use of biological recognition elements, which are expensive and easy to lose specific recognizing abilities. In addition, compared with the widely used MPN method which would take up to 15 days to accomplish the detection process, the proposed method can shorten the detection time greatly. (C) 2013 Elsevier B.V. All rights reserved.; This work presents a novel specific detection method for sulfate-reducing bacteria (SRB) based on sulfuration conversion from ZnO arrays to ZnS arrays. The sulfuration process was conducted in two steps. First, ZnO@ZnS intermediate nanocomposite arrays were formed by the sulfuration reaction between ZnO and sulfide, which is the characteristic enzymatic metabolism product of SRB. Next, unconverted ZnO cores were washed away in ammonia buffer solutions, leaving ZnS arrays on the surface of ITO substrates. The array conversion process can be used for SRB detection because the final state of ZnS arrays was highly related with the amount of accumulated sulfide, which was decided by the initial concentration of SRB. Electrochemical impedance spectroscopy has been used to optimize experimental conditions and detect SRB in aqueous solutions. This SRB detection method based on arrays conversion from ZnO to ZnS can avoid the use of biological recognition elements, which are expensive and easy to lose specific recognizing abilities. In addition, compared with the widely used MPN method which would take up to 15 days to accomplish the detection process, the proposed method can shorten the detection time greatly. (C) 2013 Elsevier B.V. All rights reserved.
关键词Detection Zno Arrays Zns Arrays Sulfate-reducing Bacteria
学科领域Chemistry ; Electrochemistry ; Instruments & Instrumentation
DOI10.1016/j.snb.2013.01.076
URL查看原文
收录类别SCI
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000317941100039
WOS关键词SENSITIZED SOLAR-CELLS ; NANOWIRE ARRAYS ; NANOSTRUCTURES ; GROWTH
WOS标题词Science & Technology ; Physical Sciences ; Technology
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/16360
专题海洋环境腐蚀与生物污损重点实验室
通讯作者Zhang, D
作者单位1.Chinese Acad Sci, Inst Oceanol, Natl Engn Res Ctr Marine Corros Protect, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所
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Qi, Peng,Zhang, Dun,Wan, Yi,et al. Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays[J]. SENSORS AND ACTUATORS B-CHEMICAL,2013,181:274-279.
APA Qi, Peng,Zhang, Dun,Wan, Yi,&Zhang, D.(2013).Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays.SENSORS AND ACTUATORS B-CHEMICAL,181,274-279.
MLA Qi, Peng,et al."Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays".SENSORS AND ACTUATORS B-CHEMICAL 181(2013):274-279.
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