IOCAS-IR
Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity
Sun Yuhao1,2; Chen Xiaolin1; Liu Song1; Yu Huahua1; Li Rongfeng1; Wang Xueqin1; Qin Yukun1; Li Pengcheng1
2018-05-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:3Pages:882-891
Corresponding AuthorChen Xiaolin(chenxl@qdio.ac.cn) ; Li Pengcheng(pcli@qdio.ac.cn)
AbstractHeparin has been used as an anticoagulant drug for many years, but it has significant side effects. In the search far good substitutes, low molecular weight (MW) polysaccharides from Sargassum fusiforme have been examined and confirmed to possess biological activities. Here, S. fusiforme polysaccharides (SFP) were extracted and subjected to a hydrogen peroxide (H2O2) oxidation method for the preparation of low-MW SFP (LSFP). The effects of temperature, pH, and H2O2 concentration on the degradation process were also examined. Several LSFP of 36, 9, 5.7, and 2.7 kDa were obtained under different conditions, and their anticoagulant activities studied in vitro. The results showed that SFP and LSFP prolonged activated partial thromboplastin (APTT), prothrombin (PT) and thrombin times (TT) significantly, indicating that these low MW polysaccharides possessed anticoagulant activity in the intrinsic, extrinsic, and common coagulation pathways. As these effects were related to the MW of the polysaccharides in APTT and TT but not in PT, the contents of the monosaccharide fucose and sulfate and the polysaccharide MW could have exerted combined effects. The details of this mechanism require further verification.
Keywordanticoagulant activity Sargassum fusiforme low molecular weight degradation
DOI10.1007/s00343-018-7089-6
Indexed BySCI
Language英语
Funding ProjectOcean Public Welfare Scientific Research Project, State Oceanic Administration of the People's Republic of China[201505033] ; Qingdao People's Livelihood Science and Technology Program[16-6-2-41-nsh]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000438791100026
PublisherSCIENCE PRESS
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163508
Collection中国科学院海洋研究所
Corresponding AuthorChen Xiaolin; Li Pengcheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun Yuhao,Chen Xiaolin,Liu Song,et al. Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(3):882-891.
APA Sun Yuhao.,Chen Xiaolin.,Liu Song.,Yu Huahua.,Li Rongfeng.,...&Li Pengcheng.(2018).Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(3),882-891.
MLA Sun Yuhao,et al."Preparation of low molecular weight Sargassum fusiforme polysaccharide and its anticoagulant activity".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.3(2018):882-891.
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