Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis | |
Yu, Bin1,4; Niu, Jianfeng1,2,3; Feng, Jianhua1; Xu, Meiling1; Xie, Xiujun1,2,3; Gu, Wenhui1,2,3; Gao, Shan1,2,3; Wang, Guangce1,2,3 | |
2018-07-25 | |
发表期刊 | FRONTIERS IN PLANT SCIENCE |
ISSN | 1664-462X |
卷号 | 9页码:13 |
通讯作者 | Niu, Jianfeng(jf_niu@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
摘要 | Pyropia yezoensis can survive the severe water loss that occurs during low tide, making it an ideal species to investigate the acclimation mechanism of intertidal seaweed to special extreme environments. In this study, we determined the effects of high salinity on photosynthesis using increasing salinity around algal tissues. Both electron transport rates, ETR (I) and ETR (II), showed continuous decreases as the salinity increased. However, the difference between these factors remained relatively stable, similar to the control. Inhibitor experiments illustrated that there were at least three different cyclic electron transport pathways. Under conditions of severe salinity, NAD(P) H could be exploited as an endogenous electron donor to reduce the plastoquinone pool in Py. yezoensis. Based on these findings, we next examined how these different cyclic electron transport (CETs) pathways were coordinated by cloning the gene (HM370553) for ferredoxin-NADP C oxidoreductase (FNR). A phylogenetic tree was constructed, and the evolutionary relationships among different FNRs were evaluated. The results indicated that the Py. yezoensis FNR showed a closer relationship with cyanobacterial FNR. The results of both real-time polymerase chain reaction and western blotting showed that the enzyme was upregulated under 90-120 h salinity. Due to the structurefunction correlations in organism, Py. yezoensis FNR was proposed to be involved in NAD(P) H-dependent Fd C reduction under severe salinity conditions. Thus, through the connection between different donors bridged by FNR, electrons were channeled toward distinct routes according to the different metabolic demands. This was expected to make the electron transfer in the chloroplasts become more flexible and to contribute greatly to acclimation of Py. yezoensis to the extreme variable environments in the intertidal zone. |
关键词 | electron transportation ferredoxin-NADP(+) reductase Pyropia yezoensis stress responding environmental acclimation |
DOI | 10.3389/fpls.2018.01092 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41776150] ; China Agriculture Research System-50 and National Key Research and Development Project[2016YFC1400600] ; Science and Technology Plan of Jiangsu Province[BE2016330] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences[XDA11020705] ; National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41776150] ; China Agriculture Research System-50 and National Key Research and Development Project[2016YFC1400600] ; Science and Technology Plan of Jiangsu Province[BE2016330] ; Strategic Leading Science and Technology Projects of Chinese Academy of Sciences[XDA11020705] |
WOS研究方向 | Plant Sciences |
WOS类目 | Plant Sciences |
WOS记录号 | WOS:000439717600001 |
出版者 | FRONTIERS MEDIA SA |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/163502 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Niu, Jianfeng; Wang, Guangce |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Yu, Bin,Niu, Jianfeng,Feng, Jianhua,et al. Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis[J]. FRONTIERS IN PLANT SCIENCE,2018,9:13. |
APA | Yu, Bin.,Niu, Jianfeng.,Feng, Jianhua.,Xu, Meiling.,Xie, Xiujun.,...&Wang, Guangce.(2018).Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis.FRONTIERS IN PLANT SCIENCE,9,13. |
MLA | Yu, Bin,et al."Regulation of Ferredoxin-NADP(+) Oxidoreductase to Cyclic Electron Transport in High Salinity Stressed Pyropia yezoensis".FRONTIERS IN PLANT SCIENCE 9(2018):13. |
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