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COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)
Lyu, Man1,3; Lei, Yanli1,3; Li, Tiegang2,3,4
2018-07-01
Source PublicationJOURNAL OF FORAMINIFERAL RESEARCH
ISSN0096-1191
Volume48Issue:3Pages:186-192
Corresponding AuthorLei, Yanli(leiyanli@qdio.ac.cn) ; Li, Tiegang()
AbstractWe carried out a series of experiments to evaluate the efficiency of preserving DNA from porcelaneous foraminifera (Quinqueloculina spp.) and a second set to assess the effect of Rose Bengal staining on molecular processing. The first experimental setup assessed three methods of DNA preservation (air-drying, freezing with or without seawater, and Guanidine lysis buffer treatment with or without EDTA (Ethylenediaminetetraacetic acid)). Our study produced the following results: 1) there were no significant differences in DNA preservation when samples were air dried across a range of temperatures (20-120 degrees C); samples frozen at -20 degrees C appeared better preserved than at those frozen at -80 degrees C, and freezing without seawater appeared to produce better preservation than with seawater, though differences in freezing treatments were not significant (p>0.05); samples in Guanidine lysis buffer with EDTA and stored at -20 degrees C were well preserved (p<0.05); 2) sometimes, DNA was successfully extracted from samples stained with Rose Bengal. We recommend Guanidine lysis buffer with EDTA, stored at -20 degrees C for up to six weeks, as the best protocol for preservation of DNA from porcelaneous foraminifera.
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA11030104] ; National Science Foundation of China[41476043] ; National Science Foundation of China[41230959] ; National Program on 'Global Change and Air-Sea Interaction'[GASI-GEOGE-06-02] ; Continental Shelf Drilling Program of China[GZH201100202] ; Paul Bronnimann Foundation
WOS Research AreaPaleontology
WOS SubjectPaleontology
WOS IDWOS:000439322600002
PublisherALLEN PRESS INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163450
Collection海洋生物分类与系统演化实验室
Corresponding AuthorLei, Yanli; Li, Tiegang
Affiliation1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China
2.SOA, Inst Oceanog 1, Key Lab Marine Sedimentol & Environm Geol, Qingdao 266061, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Geol, Qingdao 266061, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Lyu, Man,Lei, Yanli,Li, Tiegang. COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)[J]. JOURNAL OF FORAMINIFERAL RESEARCH,2018,48(3):186-192.
APA Lyu, Man,Lei, Yanli,&Li, Tiegang.(2018).COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA).JOURNAL OF FORAMINIFERAL RESEARCH,48(3),186-192.
MLA Lyu, Man,et al."COMPARISON OF DIFFERENT DNA PRESERVATION METHODS ON QUINQUELOCULINA SPP. (FORAMINIFERA, MILIOLIDA)".JOURNAL OF FORAMINIFERAL RESEARCH 48.3(2018):186-192.
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