IOCAS-IR
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis
Chi, Heng1,2,3; Sormo, Kristian Gillebo1; Diao, Jing2; Dalmo, Roy Ambli1
2019-10-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume93Pages:28-38
Corresponding AuthorChi, Heng(chiheng@qdio.ac.cn) ; Dalmo, Roy Ambli(roy.dalmo@uit.no)
AbstractEomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed in head kidney and spleen in response to live Vibrio anguillarum infection in vivo, and spleen leukocytes in vitro after PMA and poly I:C stimulation. Furthermore, we determined a 694 bp sequence, upstream of the transcriptional start site (TSS), to contain a number of sequence motifs that matched known transcription factor-binding sites, Activities of the presumptive regulatory gene were assessed by transfecting different 5'-deletion constructs in CHSE-214 cells. The results showed that the basal promoters and positive transcriptional regulator activities of GmEomes were dependent by sequences located from -694 to -376 bp upstream of TSS. Furthermore, we found that some Eomes binding sites were present in the 5'-flanking regions of the cod IFN gamma gene predicted by bioinformatics. However, Co-transfection of eomesodermin overexpression plasmids with INF gamma reporter vector into CHSE-214 cells determined that Atlantic cod eomesodermin played a minor role in activation of the INF gamma promoter.
KeywordEomesodermin Atlantic cod Molecular cloning Promoter structure Function analysis
DOI10.1016/j.fsi.2019.07.021
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31872594] ; National Natural Science Foundation of China[31402326] ; Research Council of Norway[237315] ; Research Council of Norway[239140] ; Open Fund of Shandong Key Laboratory of Disease Control in Mariculture[KF201801] ; Tromso Research Foundation ; Key Laboratory of Mariculture (KLM), Ministry of Education, OUC
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000488998300004
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/163411
Collection中国科学院海洋研究所
Corresponding AuthorChi, Heng; Dalmo, Roy Ambli
Affiliation1.UiT Arctic Univ Norway, Fac Biosci Fisheries & Econ, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway
2.Marine Biol Inst Shandong Prov, Shandong Key Lab Dis Control Mariculture, Qingdao 266104, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chi, Heng,Sormo, Kristian Gillebo,Diao, Jing,et al. T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2019,93:28-38.
APA Chi, Heng,Sormo, Kristian Gillebo,Diao, Jing,&Dalmo, Roy Ambli.(2019).T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis.FISH & SHELLFISH IMMUNOLOGY,93,28-38.
MLA Chi, Heng,et al."T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis".FISH & SHELLFISH IMMUNOLOGY 93(2019):28-38.
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