| Morphological, Physiological, and Molecular Evidences Suggest that Euplotes parawoodruffi is a Junior Synonym of Euplotes woodruffi (Ciliophora, Euplotida) | |
Dai, Renhai1,2; Xu, Kuidong1 ; He, Yingying1; Xu, KD
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| 2013 | |
| 发表期刊 | JOURNAL OF EUKARYOTIC MICROBIOLOGY
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| ISSN | 1066-5234 |
| 卷号 | 60期号:1页码:70-78 |
| 文章类型 | Article |
| 摘要 | Euplotes woodruffi Gaw 1939 is a striking ciliate species commonly used in many fields of protistology. Euplotes parawoodruffi Song and Bradbury 1997 is separated from E. woodruffi by several morphological differences (e.g. the absence of pre-oral pouch) and in particular the brackish habitat (vs. freshwater). However, recent molecular data dispute the separation of E. woodruffi into two species. Based on a brackish isolate from the southeast of China, we investigated the morphology, molecular phylogeny, and adaptation of E. woodruffi using living observation, protargol impregnation, gene sequencing, and cultivation experiments. The morphological variability present in our isolate encompasses the previous descriptions of E. woodruffi and E. parawoodruffi. The pre-oral pouch, which occurs in living cells of freshwater isolates, is also recognizable in the brackish specimens. Laboratory experiments suggested that the brackish isolate could acclimatize and reproduce in both brackish and fresh water. The molecular analyses using 13 available small subunit (SSU) rRNA gene sequences showed that the minimum pairwise sequence similarity is as high as 99.2% and nine sequences from both brackish and freshwater isolates are even 100% matched. Therefore, we conclude that E. parawoodruffi is a junior synonym of E. woodruffi.; Euplotes woodruffi Gaw 1939 is a striking ciliate species commonly used in many fields of protistology. Euplotes parawoodruffi Song and Bradbury 1997 is separated from E. woodruffi by several morphological differences (e.g. the absence of pre-oral pouch) and in particular the brackish habitat (vs. freshwater). However, recent molecular data dispute the separation of E. woodruffi into two species. Based on a brackish isolate from the southeast of China, we investigated the morphology, molecular phylogeny, and adaptation of E. woodruffi using living observation, protargol impregnation, gene sequencing, and cultivation experiments. The morphological variability present in our isolate encompasses the previous descriptions of E. woodruffi and E. parawoodruffi. The pre-oral pouch, which occurs in living cells of freshwater isolates, is also recognizable in the brackish specimens. Laboratory experiments suggested that the brackish isolate could acclimatize and reproduce in both brackish and fresh water. The molecular analyses using 13 available small subunit (SSU) rRNA gene sequences showed that the minimum pairwise sequence similarity is as high as 99.2% and nine sequences from both brackish and freshwater isolates are even 100% matched. Therefore, we conclude that E. parawoodruffi is a junior synonym of E. woodruffi. |
| 关键词 | Brackish Freshwater Phylogeny Salinity Tolerance Taxonomy |
| 学科领域 | Microbiology |
| DOI | 10.1111/jeu.12007 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| WOS记录号 | WOS:000313122500008 |
| WOS关键词 | WATER EUPLOTES ; PROTOZOA ; HYPOTRICHIDA ; REDESCRIPTION ; GENERA |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16338 |
| 专题 | 海洋生物分类与系统演化实验室 |
| 通讯作者 | Xu, KD |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Dept Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Dai, Renhai,Xu, Kuidong,He, Yingying,et al. Morphological, Physiological, and Molecular Evidences Suggest that Euplotes parawoodruffi is a Junior Synonym of Euplotes woodruffi (Ciliophora, Euplotida)[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2013,60(1):70-78. |
| APA | Dai, Renhai,Xu, Kuidong,He, Yingying,&Xu, KD.(2013).Morphological, Physiological, and Molecular Evidences Suggest that Euplotes parawoodruffi is a Junior Synonym of Euplotes woodruffi (Ciliophora, Euplotida).JOURNAL OF EUKARYOTIC MICROBIOLOGY,60(1),70-78. |
| MLA | Dai, Renhai,et al."Morphological, Physiological, and Molecular Evidences Suggest that Euplotes parawoodruffi is a Junior Synonym of Euplotes woodruffi (Ciliophora, Euplotida)".JOURNAL OF EUKARYOTIC MICROBIOLOGY 60.1(2013):70-78. |
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